Recombinant Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR14774] to TXNIP - Chimeric
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric)
See all TXNIP primary antibodies -
Description
Mouse monoclonal [EPR14774] to TXNIP - Chimeric -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate. ICC-IF: Hela, NIH-3T3 IHC-P: FFPE Human Kidney Normal, Mouse Kidney Normal
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General notes
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab188865). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14774 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa). |
IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. -
Sequence similarities
Belongs to the arrestin family. -
Post-translational
modificationsUbiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10628 Human
- Entrez Gene: 56338 Mouse
- Entrez Gene: 117514 Rat
- Omim: 606599 Human
- SwissProt: Q9H3M7 Human
- SwissProt: Q8BG60 Mouse
- SwissProt: Q5M7W1 Rat
- Unigene: 533977 Human
see all -
Alternative names
- EST01027 antibody
- HHCPA78 antibody
- THIF antibody
see all
Images
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All lanes : Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution
Predicted band size: 44 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds
Lane 2: 70 seconds
Lane 3: 50 seconds
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Ab210826 staining TXNIP in HeLa (Human cervix adenocarcinoma epithelial cell line) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with primary antibody at 1/100 dilution (1µg/ml) (red). An Alexa Fluor® 488 Goat anti mouse IgG (ab150113) was used at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as isotype control (black). Cell without incubation with primary antibody and secondary antibody (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
Ab210826 staining TXNIP in 293T (human embryonic kidney epithelial cell line) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). The cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 10µg/ml (1:100 dilution). An Alexa Fluor® 488 Goat Anti-Mouse was used as the secondary antibody at 2µg/ml (ab150113). Ab179504, Anti-beta IV Tubulin was used as a counterstain at 2µg/ml and ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as secondary antibody counterstain at 4µg/ml.
For negative control 1, primary antibody was used at a 10µg/ml and ab150080 was used as secondary antibody at 4 µg/ml. For negative control 2, ab179504 was used as a primary antibody at 2µg/ml and ab150113 was used as a secondary antibody at 2 µg/ml. DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in 293T cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
IHC image of TxNIP staining in a section of formalin fixed, paraffin embedded human normal kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab210826, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry/ Immunofluorescence - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
ab210826 stained in NIH3T3 cells. The cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210826 at 5µg/ml overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Lane 1 : Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826) at 5 µg
Lane 2 : Anti-TXNIP antibody [EPR14774] (ab188865) at 5 µg
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Lane 2 : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk (LANE 1) and 2% Bovine Serum Albumin (lane 2) before being incubated with ab210826 (lane 1) and ab188865 (lane 2) overnight at 4°C. Antibody binding was detected using an anti-mouse (lane 1) and anti-rabbit (lane 2) antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
IHC image of ab210826 staining in a section of formalin fixed, paraffin embedded mouse normal kidney, using MOM detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab210826, 2µg/ml, for 15 mins at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (ab210826)
ab210826 stained in Hela cells. The cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210826 at 5µg/ml overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab210826 has been referenced in 8 publications.
- Yu W et al. Cochlear Marginal Cell Pyroptosis Is Induced by Cisplatin via NLRP3 Inflammasome Activation. Front Immunol 13:823439 (2022). PubMed: 35529876
- Yuan Y et al. TXNIP inhibits the progression of osteosarcoma through DDIT4-mediated mTORC1 suppression. Am J Cancer Res 12:3760-3779 (2022). PubMed: 36119812
- Xue T et al. Embryonic Deletion of TXNIP in GABAergic Neurons Enhanced Oxidative Stress in PV+ Interneurons in Primary Somatosensory Cortex of Aging Mice: Relevance to Schizophrenia. Brain Sci 12:N/A (2022). PubMed: 36291328
- Mao C et al. Nicotine exacerbates atherosclerosis through a macrophage-mediated endothelial injury pathway. Aging (Albany NY) 13:7627-7643 (2021). PubMed: 33626512
- Ma X et al. Ligustrazine alleviates cyclophosphamide-induced hepatotoxicity via the inhibition of Txnip/Trx/NF-?B pathway. Life Sci 274:119331 (2021). PubMed: 33716060
- Yang C et al. Role of TXNIP/NLRP3 in sepsis-induced myocardial dysfunction. Int J Mol Med 44:417-426 (2019). PubMed: 31173172
- Wang YQ et al. Down-regulation of microRNA-138 improves immunologic function via negatively targeting p53 by regulating liver macrophage in mice with acute liver failure. Biosci Rep 39:N/A (2019). PubMed: 31152110
- Han X et al. Linarin prevents LPS-induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF-?B pathways. Int J Mol Med 42:1460-1472 (2018). PubMed: 29845284