For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/txnip-antibody-epr14774-ab188865.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TXNIP antibody [EPR14774] (ab188865)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (46)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Anti-TXNIP antibody [EPR14774] (ab188865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14774] to TXNIP
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Biochemical
Product image
Diallyl tetrasulfide, ROS inducer (ab143603)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-Bag1 antibody [Y166] (ab32109)

View more associated products

Overview

  • Product name

    Anti-TXNIP antibody [EPR14774]
    See all TXNIP primary antibodies
  • Description

    Rabbit monoclonal [EPR14774] to TXNIP
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.  For western blotting, more concentrated lysates may be required when using tissues samples. 

    Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for recommended treatment conditions and positive controls.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney and Mouse liver tissue. WB: HeLa, 293T, HAP1, MCF7, K562, THP-1, MDA-MB-231, BxPC-3, HUVEC, BxPC-3, PC-12 and NIH/3T3; Human liver, skeletal muscle and kidney tissue lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14774
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-TXNIP antibody [EPR14774] - BSA and Azide free (ab215366)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Weak detection observed in western blot when using tissue lysates compared to cell lysates. Not recommended for mouse and rat tissue samples in western blot.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Weak detection observed in western blot when using tissue lysates compared to cell lysates. Not recommended for mouse and rat tissue samples in western blot.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.
  • Sequence similarities

    Belongs to the arrestin family.
  • Post-translational
    modifications

    Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9H3M7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10628 Human
    • Entrez Gene: 56338 Mouse
    • Entrez Gene: 117514 Rat
    • Omim: 606599 Human
    • SwissProt: Q9H3M7 Human
    • SwissProt: Q8BG60 Mouse
    • SwissProt: Q5M7W1 Rat
    • Unigene: 533977 Human
    • Unigene: 410189 Mouse
    • Unigene: 475353 Mouse
    • Unigene: 2758 Rat
    see all
  • Alternative names

    • EST01027 antibody
    • HHCPA78 antibody
    • THIF antibody
    • Thioredoxin binding protein 2 antibody
    • Thioredoxin interacting protein antibody
    • Thioredoxin-binding protein 2 antibody
    • Thioredoxin-interacting protein antibody
    • TXNIP antibody
    • TXNIP_HUMAN antibody
    • Upregulated by 1,25 dihydroxyvitamin D 3 antibody
    • VDUP1 antibody
    • Vitamin D3 up-regulated protein 1 antibody
    see all

Images

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TXNIP knockout HAP1 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblasts cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab188865 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab188865 was shown to recognize TXNIP in wild-type HAP1 cells as signal was lost at the expected MW in TXNIP knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TXNIP knockout samples were subjected to SDS-PAGE. ab188865 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : THP-1 (human acute monocytic leukemia) whole cell lysates
    Lane 2 : MCF7 (human breast carcinoma) whole cell lysates
    Lane 3 : MDA-MB-231 (human mammary gland epithelial adenocarcinoma) whole cell lysates
    Lane 4 : BxPC-3 (human Pancreas adenocarcinoma) whole cell lysates
    Lane 5 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysates (negative/low expression)
    Lane 6 : Human liver lysate
    Lane 7 : Human kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM/TBST

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/5000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 25uM MG-132 for 4 hours whole cell lysates.
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM H2O2 for 1 hour whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

    MG-132 induces the expression of TXNIP (PMID: 20068034). H2O2 reduces the expression of TXNIP (PMID: 24376827).

  • Anti-TXNIP antibody [EPR14774] (ab188865)
    Anti-TXNIP antibody [EPR14774] (ab188865)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (46)

Publishing research using ab188865? Please let us know so that we can cite the reference in this datasheet.

ab188865 has been referenced in 46 publications.

  • Chen N  et al. Carbohydrate response element-binding protein regulates lipid metabolism via mTOR complex1 in diabetic nephropathy. J Cell Physiol 236:625-640 (2021). PubMed: 32583421
  • Liang Y  et al. circDCUN1D4 suppresses tumor metastasis and glycolysis in lung adenocarcinoma by stabilizing TXNIP expression. Mol Ther Nucleic Acids 23:355-368 (2021). PubMed: 33425493
  • Yan YR  et al. Chronic intermittent hypoxia-induced mitochondrial dysfunction mediates endothelial injury via the TXNIP/NLRP3/IL-1ß signaling pathway. Free Radic Biol Med 165:401-410 (2021). PubMed: 33571641
  • Mao C  et al. Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. Aging (Albany NY) 13:6156-6170 (2021). PubMed: 33578393
  • Mao C  et al. Nicotine exacerbates atherosclerosis through a macrophage-mediated endothelial injury pathway. Aging (Albany NY) 13:7627-7643 (2021). PubMed: 33626512
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-TXNIP antibody [EPR14774]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 03 2018

Western blot abreview for Anti-TXNIP antibody [EPR14774]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 03 2018

Immunohistochemistry (Frozen sections) abreview for Anti-TXNIP antibody [EPR14774]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Permeabilization
Yes - 0.3% triton in PBS 10min
Specification
brain
Blocking step
10% goat serum with 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2017

Western blot abreview for Anti-TXNIP antibody [EPR14774]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
50 µg
Specification
MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 26 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.