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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TXNIP antibody [EPR14774] (ab188865)

Reviews (4) Submit a question References (75)
Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
  • Anti-TXNIP antibody [EPR14774] (ab188865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14774] to TXNIP
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TXNIP antibody [EPR14774]
    See all TXNIP primary antibodies

  • Description

    Rabbit monoclonal [EPR14774] to TXNIP

  • Host species

    Rabbit

  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.  For western blotting, more concentrated lysates may be required when using tissues samples. 

    Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for recommended treatment conditions and positive controls.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney and Mouse liver tissue. WB: HeLa, 293T, HAP1, MCF7, K562, THP-1, MDA-MB-231, BxPC-3, HUVEC, BxPC-3, PC-12 and NIH/3T3; Human liver, skeletal muscle and kidney tissue lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Weak detection observed in western blot when using tissue lysates compared to cell lysates. Not recommended for mouse and rat tissue samples in western blot.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Weak detection observed in western blot when using tissue lysates compared to cell lysates. Not recommended for mouse and rat tissue samples in western blot.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.

  • Sequence similarities

    Belongs to the arrestin family.

  • Post-translational
    modifications

    Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EST01027 antibody
    • HHCPA78 antibody
    • THIF antibody
    • Thioredoxin binding protein 2 antibody
    • Thioredoxin interacting protein antibody
    • Thioredoxin-binding protein 2 antibody
    • Thioredoxin-interacting protein antibody
    • TXNIP antibody
    • TXNIP_HUMAN antibody
    • Upregulated by 1,25 dihydroxyvitamin D 3 antibody
    • VDUP1 antibody
    • Vitamin D3 up-regulated protein 1 antibody
    see all

Images

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TXNIP knockout HAP1 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblasts cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab188865 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab188865 was shown to recognize TXNIP in wild-type HAP1 cells as signal was lost at the expected MW in TXNIP knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TXNIP knockout samples were subjected to SDS-PAGE. ab188865 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : THP-1 (human acute monocytic leukemia) whole cell lysates
    Lane 2 : MCF7 (human breast carcinoma) whole cell lysates
    Lane 3 : MDA-MB-231 (human mammary gland epithelial adenocarcinoma) whole cell lysates
    Lane 4 : BxPC-3 (human Pancreas adenocarcinoma) whole cell lysates
    Lane 5 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysates (negative/low expression)
    Lane 6 : Human liver lysate
    Lane 7 : Human kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM/TBST

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/5000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (ab188865)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    Western blot - Anti-TXNIP antibody [EPR14774] (ab188865)
    All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 25uM MG-132 for 4 hours whole cell lysates.
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM H2O2 for 1 hour whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

    MG-132 induces the expression of TXNIP (PMID: 20068034). H2O2 reduces the expression of TXNIP (PMID: 24376827).

  • Anti-TXNIP antibody [EPR14774] (ab188865)
    Anti-TXNIP antibody [EPR14774] (ab188865)

References (75)

Publishing research using ab188865? Please let us know so that we can cite the reference in this datasheet.

ab188865 has been referenced in 75 publications.

  • Zhang M  et al. Nrf2 Signaling Pathway Mediates the Protective Effects of Daphnetin Against D-Galactose Induced-Premature Ovarian Failure. Front Pharmacol 13:810524 (2022). PubMed: 35153783
  • Yan K  et al. Leonurine inhibits the TXNIP/NLRP3 and NF-κB pathways via Nrf2 activation to alleviate carrageenan-induced pleurisy in mice. Phytother Res 36:2161-2172 (2022). PubMed: 35285100
  • Nyandwi JB  et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
  • Zhao Y  et al. Circ_SAR1A regulates the malignant behavior of lung cancer cells via the miR-21-5p/TXNIP axis. J Clin Lab Anal 36:e24366 (2022). PubMed: 35334496
  • Yao A  et al. Cytotoxic and Radiosensitising Effects of a Novel Thioredoxin Reductase Inhibitor in Brain Cancers. Mol Neurobiol 59:3546-3563 (2022). PubMed: 35344158
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-TXNIP antibody [EPR14774]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 03 2018

Western blot abreview for Anti-TXNIP antibody [EPR14774]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 03 2018

Immunohistochemistry (Frozen sections) abreview for Anti-TXNIP antibody [EPR14774]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Permeabilization
Yes - 0.3% triton in PBS 10min
Specification
brain
Blocking step
10% goat serum with 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2017

Western blot abreview for Anti-TXNIP antibody [EPR14774]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
50 µg
Specification
MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 26 2015

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