Recombinant Anti-TZP antibody [EPR25998-35] (ab285366)
![Western blot - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-476751-anti-phf20-antibody-epr2599835-western-blot-carcinoma.jpg "Western blot - Anti-TZP antibody [EPR25998-35] (ab285366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25998-35] to TZP
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TZP antibody [EPR25998-35]
See all TZP primary antibodies -
Description
Rabbit monoclonal [EPR25998-35] to TZP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt (Intra) or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT116, NCI-H1299 and F9 whole cell lysate. Mouse heart tissue lysate. ICC/IF: HCT 116 and F9 cells. IP: ab285366 IP in HCT116 and F9 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25998-35 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab285366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 115 kDa.
|
|
| IP |
1/30.
|
|
| ICC/IF |
1/1000.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 115 kDa. |
|
IP
1/30. |
|
ICC/IF
1/1000. |
Target
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Function
Possible transcription factor. -
Tissue specificity
Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues. -
Sequence similarities
Contains 1 A.T hook DNA-binding domain.
Contains 1 C2H2-type zinc finger.
Contains 1 PHD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51230 Human
- Entrez Gene: 228829 Mouse
- Omim: 610335 Human
- SwissProt: Q9BVI0 Human
- SwissProt: Q8BLG0 Mouse
- Unigene: 517044 Human
- Unigene: 427078 Mouse
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Alternative names
- C20orf104 antibody
- GLEA2 antibody
- Glioma-expressed antigen 2 antibody
see all
Images
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Western blot - Anti-TZP antibody [EPR25998-35] (ab285366)All lanes : Anti-TZP antibody [EPR25998-35] (ab285366) at 1/1000 dilution
Lane 1 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : NCI-H460 (human Large Cell Lung Cancer epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: NCI-H460 (PMID: 26722404)
Exposure time: 8 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-5-anti-phf20-antibody-epr2599835-immunocytochemistry-hct-116.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TZP antibody [EPR25998-35] (ab285366)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 cells labelling TZP with ab285366 at 1/1000 (0.589 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).
Confocal image showing nuclear staining in HCT 116 cell line.
Negative control: NCI-H460 (PMID: 26722404).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Immunoprecipitation - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-7-anti-phf20-antibody-epr2599835-immunoprecipitation-hct116.jpg)
Immunoprecipitation - Anti-TZP antibody [EPR25998-35] (ab285366)TZP was immunoprecipitated from 0.35 mg HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate 10 µg with ab285366 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285366 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HCT116 whole cell lysate 10 µg
Lane 2: ab285366 IP in HCT116 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab285366 in HCT116 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
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![Western blot - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-476805-anti-phf20-antibody-epr2599835-western-blot-carcinoma1.jpg)
Western blot - Anti-TZP antibody [EPR25998-35] (ab285366)All lanes : Anti-TZP antibody [EPR25998-35] (ab285366) at 1/1000 dilution
Lane 1 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Lane 2: This blot was developed using a higher sensitivity ECL substrate.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-6-anti-phf20-antibody-epr2599835-immunocytochemistry-f9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TZP antibody [EPR25998-35] (ab285366)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 cells labelling TZP with ab285366 at 1/1000 (0.589 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).
Confocal image showing nuclear staining in F9 cell line is observed.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
-
![Immunoprecipitation - Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-8-anti-phf20-antibody-epr2599835-immunoprecipitation-f9.jpg)
Immunoprecipitation - Anti-TZP antibody [EPR25998-35] (ab285366)TZP was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate 10 µg with ab285366 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab285366 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: F9 whole cell lysate 10 µg
Lane 2: ab285366 IP in F9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab285366 in F9 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
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![Anti-TZP antibody [EPR25998-35] (ab285366)](https://www.abcam.com/ps/products/285/ab285366/Images/ab285366-9-Benefits-of-Recombinant-antibodies.jpg)
Anti-TZP antibody [EPR25998-35] (ab285366)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab285366 has not yet been referenced specifically in any publications.