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Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

Reviews (1) Submit a question References (96)
Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

Key features and details

  • Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

Overview

  • Product name

    Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
    See all Ubiquinol-Cytochrome C Reductase Core Protein I primary antibodies

  • Description

    Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human, Cow, Rat or Mouse heart mitochondrial lysate, HepG2 mitochondrial lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
WB (1)
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 53 kDa.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 53 kDa.

Target

Images

  • Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    All lanes : Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252) at 0.5 µg/ml

    Lane 1 : Human heart mitochondrial lysate
    Lane 2 : Cow heart mitochondrial lysate
    Lane 3 : Rat heart mitochondrial lysate
    Lane 4 : Mouse heart mitochondrial lysate
    Lane 5 : HepG2 mitochondrial lysate

    Predicted band size: 53 kDa



    Extra bands in the Mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

    ab110252 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Overlay histogram showing HepG2 cells stained with ab110252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References (96)

Publishing research using ab110252? Please let us know so that we can cite the reference in this datasheet.

ab110252 has been referenced in 96 publications.

  • Zhang Z  et al. Ruthenium 360 and mitoxantrone inhibit mitochondrial calcium uniporter channel to prevent liver steatosis induced by high-fat diet. Br J Pharmacol 179:2678-2696 (2022). PubMed: 34862596
  • Robinson DRL  et al. Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain. Front Cell Dev Biol 10:786268 (2022). PubMed: 35300415
  • Jackson TD  et al. Sideroflexin 4 is a complex I assembly factor that interacts with the MCIA complex and is required for the assembly of the ND2 module. Proc Natl Acad Sci U S A 119:e2115566119 (2022). PubMed: 35333655
  • Zhao MY  et al. Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro. Front Pharmacol 13:816432 (2022). PubMed: 35517791
  • Liu W  et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun 13:2748 (2022). PubMed: 35585086
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Isolated Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 05 2011

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