Anti-Ubiquitin antibody (ab19247)
Key features and details
- Rabbit polyclonal to Ubiquitin
- Suitable for: WB
- Reacts with: Mouse, Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Ubiquitin antibody
See all Ubiquitin primary antibodies -
Description
Rabbit polyclonal to Ubiquitin -
Host species
Rabbit -
Specificity
It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 10 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 10 kDa. |
Target
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Relevance
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains. -
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- SwissProt: P0CG47 Human
- SwissProt: P0CG48 Human
- SwissProt: P62979 Human
- SwissProt: P62987 Human
- SwissProt: P62988 Human
- SwissProt: P0CG49 Mouse
see all -
Alternative names
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
see all
Images
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Western blot - Anti-Ubiquitin antibody (ab19247)All lanes :
Lane 1 : HeLa cell lysate (Control, untreated)
Lane 2 : HeLa cells treated with MG132 (50 uM for 90 min)
Lane 3 : Mouse Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 10 kDaThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab19247 and ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.
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Western blot - Anti-Ubiquitin antibody (ab19247)Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution + Heat-shocked HeLa cell lysate
Predicted band size: 10 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (53)
ab19247 has been referenced in 53 publications.
- Schneider KL et al. Using reporters of different misfolded proteins reveals differential strategies in processing protein aggregates. J Biol Chem 298:102476 (2022). PubMed: 36096201
- Wolfe R et al. ITCH deficiency clinical phenotype expansion and mitochondrial dysfunction. Mol Genet Metab Rep 33:100932 (2022). PubMed: 36338154
- Heo G et al. Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes. PLoS One 17:e0277477 (2022). PubMed: 36441709
- Abe H et al. Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation. Nat Commun 13:7212 (2022). PubMed: 36443288
- Panagaki D et al. Nuclear envelope budding is a response to cellular stress. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34290138