Recombinant Anti-UFC1 antibody [EPR15014-102] (ab189252)
![Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-450245-anti-ufc1-antibody-epr15014-102-western-blot-hek-293t-lysates.jpg "Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15014-102] to UFC1
- Suitable for: IP, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UFC1 antibody [EPR15014-102]
See all UFC1 primary antibodies -
Description
Rabbit monoclonal [EPR15014-102] to UFC1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver tissue lysate and HEK-293T, HAP1, U87-MG, MCF7, A549, RAW 264.7, and NIH 3T3 cell lysates. IHC-P: Human colon and human transitional cell carcinoma of bladder tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15014-102 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/50.
|
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| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IP
1/50. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 51506 Human
- Omim: 610554 Human
- SwissProt: Q9Y3C8 Human
- Unigene: 301412 Human
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Alternative names
- CGI-126 antibody
- HSPC155 antibody
- Ubiquitin fold modifier conjugating enzyme 1 antibody
see all
Images
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Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : UFC1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab189252 observed at 20 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab189252 was shown to react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type HEK-293T and UFC1 HEK-293T KO cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189252 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-224607-anti-ufc1-antibody-epr15014-102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-384994-anti-ufc1-antibody-epr15014-102-western-blot-hap1-wt-hap1-ufc1-knockout-mcf7-a549.jpg)
Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : UFC1 knockout HAP1 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab189252 observed at 20 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab189252 was shown to react with UFC1 in wild-type HAP1 cells in western blot. Loss of signal was observed when UFC1 knockout sample was used. Wild-type HAP1 and UFC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab189252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-224609-anti-ufc1-antibody-epr15014-102-western-blot.jpg)
Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/20000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : U87-MG cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 19 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-224606-anti-ufc1-antibody-epr15014-102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-224635-anti-ufc1-antibody-epr15014-102-immunoprecipitation.jpg)
Immunoprecipitation - Anti-UFC1 antibody [EPR15014-102] (ab189252)Western blot analysis of immunoprecipitation pellet from MCF7 cell lysate immunoprecipitated using ab189252 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-UFC1 antibody [EPR15014-102] (ab189252)](https://www.abcam.com/ps/products/189/ab189252/Images/ab189252-8-benefits-of-recombinant-antibodies.png)
Anti-UFC1 antibody [EPR15014-102] (ab189252)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab189252 has been referenced in 2 publications.
- Qin B et al. STK38 promotes ATM activation by acting as a reader of histone H4 ufmylation. Sci Adv 6:eaax8214 (2020). PubMed: 32537488
- Qin B et al. UFL1 promotes histone H4 ufmylation and ATM activation. Nat Commun 10:1242 (2019). PubMed: 30886146