Recombinant Anti-UGT1A1 antibody [EPR9592] (ab170858)
![Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)](https://www.abcam.com/ps/products/170/ab170858/Images/ab170858-261571-anti-ugt1a1-antibody-epr9592-western-blot.jpg "Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9592] to UGT1A1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UGT1A1 antibody [EPR9592]
See all UGT1A1 primary antibodies -
Description
Rabbit monoclonal [EPR9592] to UGT1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human UGT1A1 (Cysteine residue). The exact sequence is proprietary.
Database link: P22309 -
Positive control
- Human fetal liver and LoVo lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.16% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9592 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
- Information by UniProt
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Database links
- Entrez Gene: 54658 Human
- Omim: 191740 Human
- SwissProt: P22309 Human
- Unigene: 554822 Human
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Alternative names
- BILIQTL1 antibody
- Bilirubin specific UDPGT isozyme 1 antibody
- bilirubin UDP glucuronosyltransferase 1 1 antibody
see all
Images
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Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution + LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 60 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)](https://www.abcam.com/ps/products/170/ab170858/Images/ab170858-170692-anti-ugt1a1-antibody-epr9592-western-blot.jpg)
Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)All lanes : Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : LoVo lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
![Anti-UGT1A1 antibody [EPR9592] (ab170858)](https://www.abcam.com/ps/products/170/ab170858/Images/ab170858-3-benefits-of-recombinant-antibodies.png)
Anti-UGT1A1 antibody [EPR9592] (ab170858)
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab170858 has been referenced in 9 publications.
- Asai Y et al. The Effect of Single-Walled Carbon Nanotubes on UDP-Glucuronosyltransferase 1A Activity in Human Liver. Biol Pharm Bull 45:446-451 (2022). PubMed: 35370268
- van der Schoor LWE et al. Potential of therapeutic bile acids in the treatment of neonatal Hyperbilirubinemia. Sci Rep 11:11107 (2021). PubMed: 34045606
- Paszek M & Tukey RH NRF2-Independent Regulation of Intestinal Constitutive Androstane Receptor by the Pro-Oxidants Cadmium and Isothiocyanate in hUGT1 Mice. Drug Metab Dispos 48:25-30 (2020). PubMed: 31704714
- Hansmann E et al. Differential Role of Liver X Receptor (LXR) a and LXRß in the Regulation of UDP-Glucuronosyltransferase 1A1 in Humanized UGT1 Mice. Drug Metab Dispos 48:255-263 (2020). PubMed: 31980500
- Gu C et al. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. J Cell Mol Med 23:4165-4178 (2019). PubMed: 30950200
- Štampar M et al. Development of in vitro 3D cell model from hepatocellular carcinoma (HepG2) cell line and its application for genotoxicity testing. Arch Toxicol 93:3321-3333 (2019). PubMed: 31542801
- Chen S et al. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440 (2017). PubMed: 28167773
- Nie YL et al. Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1. Drug Metab Dispos 45:1372-1378 (2017). PubMed: 29025858
- Chow EC et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). PubMed: 27342868