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RecombinantRabMAb

Recombinant Anti-UHRF1 antibody [EPR18803] (ab194236)

Reviews (2) Submit a question References (2)
Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (ab194236)
  • Anti-UHRF1 antibody [EPR18803] (ab194236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18803] to UHRF1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-UHRF1 antibody [EPR18803]
    See all UHRF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18803] to UHRF1

  • Host species

    Rabbit

  • Specificity

    UHRF1 is aboundant in tissues like thymus, bone marrow. For other tissues with low expression level, please upload higher amount of tissue lysate or use lower antibody dilution.

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human thymus, colon and breast lysates; HeLa, Jurkat, U-937 and HepG2 whole cell lysates. IHC-P: Human tonsil and colon tissues; Rat spleen tissue. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 90 kDa).
IP
1/20.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 90 kDa).
IP
1/20.

Target

  • Function

    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

  • Tissue specificity

    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.

  • Developmental stage

    Expressed in fetal thymus, liver and kidney.

  • Domain

    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.

  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ac2-121 antibody
      • AL022808 antibody
      • E3 ubiquitin-protein ligase UHRF1 antibody
      • EC 6.3.2.- antibody
      • FLJ21925 antibody
      • hNP95 antibody
      • hUHRF1 antibody
      • HuNp95 antibody
      • ICBP90 antibody
      • Inverted CCAAT box binding protein of 90 kDa antibody
      • Inverted CCAAT box binding protein, 90-kD antibody
      • Inverted CCAAT box-binding protein of 90 kDa antibody
      • Liver regeneration-related protein LRRG126 antibody
      • MGC138707 antibody
      • NP95 antibody
      • Nuclear phosphoprotein 95 antibody
      • Nuclear phosphoprotein, 95-KD antibody
      • Nuclear protein 95 antibody
      • Nuclear zinc finger protein Np95 antibody
      • RING finger protein 106 antibody
      • RNF106 antibody
      • TDRD22 antibody
      • Transcription factor ICBP90 antibody
      • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
      • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
      • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
      • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
      • Ubiquitin-like with PHD and ring finger domains 1 antibody
      • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
      • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
      • UHRF1 antibody
      • UHRF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

      Lane 1 : Human colon tissue lysate
      Lane 2 : Human breast tissue lysate
      Lane 3 : Human thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

      Predicted band size: 90 kDa
      Observed band size: 30-70,90-100 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      UHRF1 has two isoforms.

      UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID: 23297342, PMID: 36593255)

    • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human kidney tissue lysate
      Lane 3 : Human spleen tissue lysate
      Lane 4 : Human colon tissue lysate
      Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 7 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

      Predicted band size: 90 kDa
      Observed band size: 30-70,90-100 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      ab181602 was used as GAPDH loading control.

      UHRF1 has two isoforms.

      UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID: 23297342, PMID: 36593255)

    • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
      All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/5000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 90 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1 and 2: 10 seconds; Lane 3: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on lymphocytes of Human tonsil is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on the epithelial cells of Human colon is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on lymphocytes of rat spleen is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (ab194236)
      Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (ab194236)

      UHRF1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab194236 at 1/20 dilution.

      Western blot was performed from the immunoprecipitate using ab194236 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: HepG2 whole cell lysate 10ug (Input).

      Lane 2: ab194236 IP in HepG2 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194236 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Anti-UHRF1 antibody [EPR18803] (ab194236)
      Anti-UHRF1 antibody [EPR18803] (ab194236)

    References (2)

    Publishing research using ab194236? Please let us know so that we can cite the reference in this datasheet.

    ab194236 has been referenced in 2 publications.

    • Saeki N  et al. Epigenetic regulator UHRF1 orchestrates proinflammatory gene expression in rheumatoid arthritis in a suppressive manner. J Clin Invest 132:N/A (2022). PubMed: 35472067
    • Yang Y  et al. Overexpression of UHRF1 and its potential role in the development of invasive ductal breast cancer validated by integrative bioinformatics and immunohistochemistry analyses. Transl Cancer Res 8:1086-1096 (2019). PubMed: 35116851

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    1-2 of 2 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UHRF1 antibody [EPR18803]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA or Citrate
    Permeabilization
    No
    Specification
    Mouse Liver
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 18 2019

    ChIP abreview for Anti-UHRF1 antibody [EPR18803]

     Excellent
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (T-lymphotropic cell line)
    Negative control
    IgG
    Specification
    T-lymphotropic cell line
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldéhyde 1%
    Positive control
    known UHRF1 binding region
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 31 2016

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