Recombinant Anti-UHRF1 antibody [EPR25061-269] (ab303552)
![Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-492161-anti-uhrf1-antibody-epr25061-269-western-blot-mouse-tissues.jpg "Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25061-269] to UHRF1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra), IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-UHRF1 antibody [EPR25061-269]
See all UHRF1 primary antibodies -
Description
Rabbit monoclonal [EPR25061-269] to UHRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra), IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Tissue lysates: Mouse thymus; Whole cell lysates: F9 (mouse embryonal carcinoma epithelial cell), ES-D3 (mouse embryonic multipotent stem Cell), C2C12 (mouse myoblasts myoblast). ICC/IF: F9 (mouse embryonal carcinoma epithelial cell). Flow cyt. intr.: F9 (mouse embryonal carcinoma epithelial cell). IP: F9 cell lysates and mouse thymus tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25061-269 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab303552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
|
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| ICC/IF |
1/500.
|
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| Flow Cyt (Intra) |
1/500.
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). |
|
ICC/IF
1/500. |
|
Flow Cyt (Intra)
1/500. |
|
IP
1/30. |
Target
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Function
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. -
Tissue specificity
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain. -
Developmental stage
Expressed in fetal thymus, liver and kidney. -
Domain
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18140 Mouse
- SwissProt: Q8VDF2 Mouse
- Unigene: 42196 Mouse
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Alternative names
- Ac2-121 antibody
- AL022808 antibody
- E3 ubiquitin-protein ligase UHRF1 antibody
see all
Images
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Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)All lanes : Anti-UHRF1 antibody [EPR25061-269] (ab303552) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Low expression: brain (PMID: 9880673)
The identity of the lower MW band at approximately 37 kDa is unknown. -
![Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-492159-anti-uhrf1-antibody-epr25061-269-western-blot-mouse-cell-lines.jpg)
Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)All lanes : Anti-UHRF1 antibody [EPR25061-269] (ab303552) at 1/1000 dilution
Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lane 2 : ES-D3 (mouse embryonic multipotent stem Cell), whole cell lysate
Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 36 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-2: 37 seconds
Lane 3: 92 secondsThe identity of the bands between 30 kDa and 50 kDa is unknown.
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![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-3-anti-uhrf1-antibody-epr25061-269-immunocytochemistry-f9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR25061-269] (ab303552)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling UHRF1 with AB303552 at 1/500 dilution (1.066 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in F9 cell line.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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![Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-2-anti-uhrf1-antibody-epr25061-269-flow-cytometry-f9.jpg)
Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR25061-269] (ab303552)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling UHRF1 with AB303552 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-4-anti-uhrf1-antibody-epr25061-269-immunoprecipitation-f9.jpg)
Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552)UHRF1 was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate with ab303552 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303552 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate 10 μg (Inset)
Lane 2: ab303552 IP in F9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab303552 in F9 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 111 seconds.
Observed MW (kDa): 95.
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![Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-5-anti-uhrf1-antibody-epr25061-269-immunoprecipitation-mouse-thymus-tissue-lysate.jpg)
Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552)UHRF1 was immunoprecipitated from 0.35 mg mouse thymus tissue lysate 10 ug with ab303552 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303552 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: mouse thymus tissue lysate 10 µg (Inset)
Lane 2: ab303552 IP in mouse thymus tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab303552 in mouse thymus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 111 seconds.
Observed MW (kDa): 95.
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![Anti-UHRF1 antibody [EPR25061-269] (ab303552)](https://www.abcam.com/ps/products/303/ab303552/Images/ab303552-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-UHRF1 antibody [EPR25061-269] (ab303552)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab303552 has not yet been referenced specifically in any publications.