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Anti-ULK2 antibody (ab97695)

Reviews (1)Q&A (7)References (8)
Western blot - Anti-ULK2 antibody (ab97695)
  • Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (ab97695)

Key features and details

  • Rabbit polyclonal to ULK2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ULK2 antibody

  • Description

    Rabbit polyclonal to ULK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 693 and 938 of Human ULK2 (NP_055498).

  • Positive control

    • 293T, H1299, HeLa, Molt-4, Raji cell lysates. HepG2 whole cell lysates. HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated.
  • Information by UniProt

  • Database links

    • Alternative names

      • ATG1B antibody
      • KIAA0623 antibody
      • Serine/threonine protein kinase ULK2 antibody
      • Serine/threonine-protein kinase ULK2 antibody
      • ULK2 antibody
      • ULK2_HUMAN antibody
      • Unc 51 (C. elegans) like kinase 2 antibody
      • Unc 51 like autophagy activating kinase 2 antibody
      • Unc 51 like kinase 2 antibody
      • Unc-51-like kinase 2 antibody
      • Unc51.2 antibody
      see all

    Images

    • Western blot - Anti-ULK2 antibody (ab97695)
      Western blot - Anti-ULK2 antibody (ab97695)
      Anti-ULK2 antibody (ab97695) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 113 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (ab97695)
      Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (ab97695)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97695 at 1/200 dilution. Lower image is merged with DNA probe.

    References (8)

    Publishing research using ab97695? Please let us know so that we can cite the reference in this datasheet.

    ab97695 has been referenced in 8 publications.

    • Li J  et al. Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy. Nat Commun 13:1172 (2022). PubMed: 35246531
    • Wang J  et al. LncRNA GAS5 Suppressed Proliferation and Promoted Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting MiR-26a-5p and Modifying ULK2. Cancer Manag Res 13:871-887 (2021). PubMed: 33551645
    • Schmitz K  et al. An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis. Nucleic Acids Res 49:6437-6455 (2021). PubMed: 34096600
    • Li G  et al. Long non-coding RNA growth arrest-specific 5 (GAS5) acts as a tumor suppressor by promoting autophagy in breast cancer. Mol Med Rep 22:2460-2468 (2020). PubMed: 32705220
    • Corona Velazquez A  et al. Poliovirus induces autophagic signaling independent of the ULK1 complex. Autophagy 14:1201-1213 (2018). PubMed: 29929428
    • Blessing AM  et al. Transcriptional regulation of core autophagy and lysosomal genes by the androgen receptor promotes prostate cancer progression. Autophagy 13:506-521 (2017). PubMed: 27977328
    • Puto LA  et al. Transcriptional Repressor DAXX Promotes Prostate Cancer Tumorigenicity via Suppression of Autophagy. J Biol Chem 290:15406-20 (2015). WB ; Human . PubMed: 25903140
    • Konno H  et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). PubMed: 24119841

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-ULK2 antibody

     Below Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (cortex)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    cortex
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 11 2017

    Question

    Thanks for the mail. Customer would prefer to exchange with anti-ULK2 (Abcam, ab56736) for ULK2,  ab115528   for NPTX2 and ab111111  for NUCKS1.  So please add ab111111 & ab56736        

    Read More
    Answer

    Thank you for your reply, and for your patience in resolving this issue. I have asked that these three replacement vials be added to your next shipment. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Question

    Thank you for your response. Enclosed is the copy of the order.

    Read More
    Answer

    I have processed your request to receive ab56736 as a replacement for ab97695. You r new order number is ******.

    Read More

    Question

     Dear John, please find enclosed scans of western blots with abcam antibody ab97695 against ULK2 – as you see there are many bands independently of how we process the IB. I would  like to try the monoclonal antibody to ULK2 that you have – 56736. Can you please send us a sample for replacement.  Western shows total lysates of  A498 renal cancer cells.

    Read More
    Answer

    I am sorry that the protocol tips did not prove to be successful and I would be happy to send you ab56736 as a replacement. I would just like to confirm the original order information. Can you confirm that it was ordered by yourself on*******. I just like to confirm that I have the original details to make sure there are no mistakes.

    Read More

    Question

    Thank you . Can you please arrange to add this in any of our order ?   Regards  

    Read More
    Answer

    Thank you for your reply. I would be happy to send replacements. Does the customer wish to try fresh vials of the antibodies they have already tried, or would they prefer different antibodies to the targets NPTX2, NUCKS1, and ULK2? I look forward to hearing from you, and I will send these replacements out promptly.

    Read More

    Question

    1) Sample prep: whole cell extracts Lysis buffer: NP40 homogenization buffer: 50mM Tris-HCl pH 8, 50mM NaF, 137 mM NaCl, 1mM EDTA, 1M NaOrthovanadate, 10ug/ml Leupeptin, 10ug/ml aprotinin, 1mM PMSF, 10% glycerol, 1% NP-40 Protease/phosphatase inhibitors: see buffer composition Boiling for ≥5 min? yes/no: boiling 5 min Protein loaded ug/lane or cells/lane: 20 ug 2) Percentage of gel : 10 % Blocking agent and concentration: 5% milk in TBS1x + tween 0.5% Blocking time: 1 hour Blocking temperature: room temp 3) Primary antibody: ULK2 polyclonal from Abcam Concentration or dilution: 1:1000 Diluent buffer: Filtered 1xPBS + 1% BSA + 0.1% NaN3 Incubation time: Overnight Incubation temperature: cold room, 4*C 4) Secondary antibody: Rabbit Concentration or dilution: 1:5000 Diluent buffer: 5% milk in wash buffer (TBS 1x + tween 0.5%) Incubation time: 1 hour Incubation temperature: Room temperature Fluorochrome or enzyme conjugate: HRP 5) Washing after primary and secondary antibodies: Buffer: TBS 1X containing 0.5% tween Number of washes: 3 If you cannot improve on my results, then kindly refund.

    Read More
    Answer

    Thank you for contacting us and for confirming the details of your experiment. I am sorry that this antibody did not perform as stated on the datasheet and your protocol seems fine. This amount will be refunded to the credit card used to make this purchase. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

    Read More

    Question

    Here is the reply from customer.     Thanks for the mail. We have tried all the steps suggested by abcam, but it did not help.   

    Read More
    Answer

    Thank you for your reply. I would be happy to offer the customer a free of charge replacement or credit. Please let me know how the customer would like to proceed and if they would like a replacement, which product they would like to receive. I would be happy to help you further once I have this additional information.

    Read More

    Question

    Hope you are fine.   Here is the reply from the customer : Dear   We have used human cancer cell lines and also the over expressing cells. We have run large gel so 100ug is not high, and as I have send in first mail, the antibody was very nonspecific and gives many bands.   Thanks Regards 

    Read More
    Answer

    Thanks for your reply and for sending the additional information. I do have a couple of additional suggestions, however I am not sure if the customer has tried these steps already. For the antibodies ab97695, ab69858, and ab7770, we would recommend diluting around 1:2000-1:3000. We would also recommend blocking the membrane in 5% BSA overnight to reduce non-specific binding. If the customer has already tried similar optimization steps, I would be happy to send replacement antibodies or alternatively issue a credit note. We would also be very grateful if the customer could provide an image of the results so that we may investigate the problem with these antibodies. I look forward to hearing from you. Please let me know if you have any questions.

    Read More

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