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RecombinantRabMAb

Recombinant Anti-UMOD antibody [EPR20071] (ab207170)

Reviews (3) Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)
  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)
  • Anti-UMOD antibody [EPR20071] (ab207170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20071] to UMOD
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-UMOD antibody [EPR20071]
    See all UMOD primary antibodies

  • Description

    Rabbit monoclonal [EPR20071] to UMOD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human urine and fetal kidney lysate; mouse and rat kidney lysates. IHC-P: Human, mouse and rat kidney tissues. IP: Human kidney lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20071

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 70 kDa).
Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 70 kDa).

Target

  • Function

    Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity.

  • Tissue specificity

    Synthesized by kidney. Most abundant protein in normal human urine.

  • Involvement in disease

    Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
    Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
    Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability.

  • Sequence similarities

    Contains 3 EGF-like domains.
    Contains 1 ZP domain.

  • Cellular localization

    Cell membrane. Secreted. Secreted after cleavage in the urine.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADMCKD2 antibody
    • FJHN antibody
    • HNFJ antibody
    • HNFJ1 antibody
    • MCKD2 antibody
    • medullary cystic kidney disease 2 (autosomal dominant) antibody
    • Tamm Horsfall glycoprotein antibody
    • Tamm Horsfall urinary glycoprotein antibody
    • Tamm-Horsfall urinary glycoprotein antibody
    • THGP antibody
    • THP antibody
    • Umod antibody
    • Urehd1 antibody
    • urehr4 antibody
    • UROM_HUMAN antibody
    • uromodulin (uromucoid, Tamm-Horsfall glycoprotein) antibody
    • Uromodulin antibody
    • Uromodulin, secreted form antibody
    • Uromucoid antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
    Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
    All lanes : Anti-UMOD antibody [EPR20071] (ab207170) at 1/10000 dilution

    Lane 1 : Human urine
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 70 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID: 18310901).

  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
    Western blot - Anti-UMOD antibody [EPR20071] (ab207170)
    All lanes : Anti-UMOD antibody [EPR20071] (ab207170) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 70 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID: 18310901).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted tubules of mouse kidney is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted tubules of rat kidney is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)
    Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    UMOD was immunoprecipitated from 0.35 mg of human kidney lysate with ab207170  at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human kidney lysate 10µg (Input).

    Lane 2: ab207170 IP in human kidney lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)
    Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    UMOD was immunoprecipitated from 0.35 mg of mouse kidney lysate with ab207170  at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human kidney lysate 10µg (Input).

    Lane 2: ab207170 IP in human kidney lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

  • Anti-UMOD antibody [EPR20071] (ab207170)
    Anti-UMOD antibody [EPR20071] (ab207170)

References (5)

Publishing research using ab207170? Please let us know so that we can cite the reference in this datasheet.

ab207170 has been referenced in 5 publications.

  • Yang HC  et al. Single-step equipment-free extracellular vesicle concentration using super absorbent polymer beads. J Extracell Vesicles 10:e12074 (2021). PubMed: 33664938
  • Neumann EK  et al. Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence. STAR Protoc 2:100747 (2021). PubMed: 34430920
  • Tang C  et al. Auxiliary genetic analysis in a Chinese adolescent NPH family by single nucleotide polymorphism screening. Mol Med Rep 21:1115-1124 (2020). PubMed: 31922211
  • Nespoux J  et al. Gene knockout of the Na+-glucose cotransporter SGLT2 in a murine model of acute kidney injury induced by ischemia-reperfusion. Am J Physiol Renal Physiol 318:F1100-F1112 (2020). PubMed: 32116018
  • Plotkin M  et al. A Uromodulin Mutation Drives Autoimmunity and Kidney Mononuclear Phagocyte Endoplasmic Reticulum Stress. Am J Pathol 190:2436-2452 (2020). PubMed: 32926855

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

IHC - Wholemount abreview for Anti-UMOD antibody [EPR20071]

 Excellent
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (Kidney)
Specification
Kidney
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Daniyal Jafree

Verified customer

Submitted May 28 2021

Flow Cytometry abreview for Anti-UMOD antibody [EPR20071]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Kidney)
Permeabilization
No
Gating Strategy
Cells should have higher values of FSC-A and higher values of Fluorescence. We performed an Assay with THP, prim. AB and sec. AB only, to see which values can be excluded.
Specification
Kidney
Preparation
Cell harvesting/tissue preparation method: Kidneys were frozen in liquid nitrogen. After that, they have been stored in -80°C. Frozen Kidneys were cut into small pieces. Fat and other tissues have been removed beforehand. Minced Kidney were incubated with Kollagenase IV. Tissue is then mechanically crushed in cell sieves (100, 70, 30 µm).
Sample buffer: 0,05 M HEPES, 1% BSA in DMEM
Fixation
none
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Boris Mo

Verified customer

Submitted Jul 07 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UMOD antibody [EPR20071]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0
Permeabilization
Yes - 0.3%Tween in PBS
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Timo Rademakers

Verified customer

Submitted Oct 01 2018

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