Recombinant Anti-UQCRB antibody [EPR15591] (ab190360)
![Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-226173-anti-uqcrb-antibody-epr15591-western-blot.jpg "Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15591] to UQCRB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-UQCRB antibody [EPR15591]
See all UQCRB primary antibodies -
Description
Rabbit monoclonal [EPR15591] to UQCRB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, Human fetal heart, 293, mouse heart, rat brain and NIH-3T3 lysates, Human adenocarcinoma of colon tissue, HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15591 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/250. |
Target
-
Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping. -
Involvement in disease
Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance. -
Sequence similarities
Belongs to the UQCRB/QCR7 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 7381 Human
- Entrez Gene: 67530 Mouse
- Entrez Gene: 362897 Rat
- Omim: 191330 Human
- SwissProt: P14927 Human
- SwissProt: Q9D855 Mouse
- Unigene: 131255 Human
-
Alternative names
- Complex III subunit 7 antibody
- Complex III subunit VII antibody
- Cytochrome b-c1 complex subunit 7 antibody
see all
Images
-
Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa -
![Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-226172-anti-uqcrb-antibody-epr15591-western-blot.jpg)
Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)Anti-UQCRB antibody [EPR15591] (ab190360) at 1/5000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa -
![Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-226171-anti-uqcrb-antibody-epr15591-western-blot.jpg)
Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-226170-anti-uqcrb-antibody-epr15591-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] (ab190360)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-226169-anti-uqcrb-antibody-epr15591-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] (ab190360)Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI. Negative controls also shown.
-
![Anti-UQCRB antibody [EPR15591] (ab190360)](https://www.abcam.com/ps/products/190/ab190360/Images/ab190360-6-benefits-of-recombinant-antibodies.png)
Anti-UQCRB antibody [EPR15591] (ab190360)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (6)
ab190360 has been referenced in 6 publications.
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Zhang Z et al. Hepatic HuR modulates lipid homeostasis in response to high-fat diet. Nat Commun 11:3067 (2020). PubMed: 32546794
- Song W et al. Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue. PLoS Biol 17:e2006571 (2019). PubMed: 30653498
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Cichocki F et al. ARID5B regulates metabolic programming in human adaptive NK cells. J Exp Med 215:2379-2395 (2018). PubMed: 30061358
- Pérez-Pérez R et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . PubMed: 27545886