Recombinant Anti-UQCRC2 antibody [EPR13051] (ab203832)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13051] to UQCRC2
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-UQCRC2 antibody [EPR13051]
See all UQCRC2 primary antibodies -
Description
Rabbit monoclonal [EPR13051] to UQCRC2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HepG2, HEK-293, Jurkat, C6 and PC-12 cell lysates; Mouse heart, rat brain and rat kidney lysates. IHC-P: Human liver tissue. ICC/IF: HepG2 and Jurkat cells. Flow Cyt (intra): Jurkat cells. IP: HepG2 and HEK-293 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13051 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (1) |
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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ICC/IF |
1/300.
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IP |
1/50.
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Notes |
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Flow Cyt (Intra)
1/150. |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ICC/IF
1/300. |
IP
1/50. |
Target
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Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex. -
Sequence similarities
Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7385 Human
- Entrez Gene: 67003 Mouse
- Entrez Gene: 293448 Rat
- Omim: 191329 Human
- SwissProt: P22695 Human
- SwissProt: Q9DB77 Mouse
- SwissProt: P32551 Rat
- Unigene: 528803 Human
see all -
Alternative names
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
see all
Images
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All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat kidney lysate
Lane 4 : C6 (Rat glial tumor cells) cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (ab203832)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRC2 with ab203832 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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UQCRC2 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HepG2 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HepG2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second. -
UQCRC2 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (16)
ab203832 has been referenced in 16 publications.
- Tan HWS et al. A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery. Nat Commun 13:3720 (2022). PubMed: 35764633
- Zhao W et al. Estrogen Deficiency Induces Mitochondrial Damage Prior to Emergence of Cognitive Deficits in a Postmenopausal Mouse Model. Front Aging Neurosci 13:713819 (2021). PubMed: 34335235
- Zhang X et al. Trib1 deficiency causes brown adipose respiratory chain depletion and mitochondrial disorder. Cell Death Dis 12:1098 (2021). PubMed: 34811364
- Bertan F et al. Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution. Cell Rep Methods 1:100002 (2021). PubMed: 35474694
- Zhang Q et al. Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. Nucleic Acids Res 49:4689-4704 (2021). PubMed: 33836087