Name: Anti-UQCRC2 antibody [EPR13051]
Brand: RabMAb
SKU: ab203832
Rating: 5 (1 reviews)

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Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13051] to UQCRC2
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Alkaline Phosphatase APC Carrier Free HRP PE

Product name

Anti-UQCRC2 antibody [EPR13051]
See all UQCRC2 primary antibodies
Description

Rabbit monoclonal [EPR13051] to UQCRC2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HepG2, HEK-293, Jurkat, C6 and PC-12 cell lysates; Mouse heart, rat brain and rat kidney lysates. IHC-P: Human liver tissue. ICC/IF: HepG2 and Jurkat cells. Flow Cyt (intra): Jurkat cells. IP: HepG2 and HEK-293 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13051
Isotype

IgG
Research areas

Alternative Versions
- Anti-UQCRC2 antibody [EPR13051] - BSA and Azide free (ab240368)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human UQCRC2 protein (denatured) (ab134532)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/150.
IHC-P		1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(1)	1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF		1/300.
IP		1/50.

Notes
Flow Cyt (Intra) 1/150.
IHC-P 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF 1/300.
IP 1/50.

Function

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
Sequence similarities

Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
Cellular localization

Mitochondrion inner membrane.
Target information above from: UniProt accession P22695 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7385 Human
- Entrez Gene: 67003 Mouse
- Entrez Gene: 293448 Rat
- Omim: 191329 Human
- SwissProt: P22695 Human
- SwissProt: Q9DB77 Mouse
- SwissProt: P32551 Rat
- Unigene: 528803 Human
- Unigene: 334206 Mouse
- Unigene: 2334 Rat
see all
Alternative names
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
- Cytochrome b-c1 complex subunit 2 antibody
- EC 1.10.2.2 antibody
- MC3DN5 antibody
- mitochondrial antibody
- QCR2 antibody
- QCR2_HUMAN antibody
- Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
- Ubiquinol cytochrome c reductase core protein II antibody
- Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
- UQCR2 antibody
- Uqcrc2 antibody
see all

Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)

All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)

All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)

All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat kidney lysate
Lane 4 : C6 (Rat glial tumor cells) cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRC2 with ab203832 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

UQCRC2 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HepG2 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

UQCRC2 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Anti-UQCRC2 antibody [EPR13051] (ab203832)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab203832? Please let us know so that we can cite the reference in this datasheet.

ab203832 has been referenced in 16 publications.

Tan HWS et al. A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery. Nat Commun 13:3720 (2022). PubMed: 35764633
Zhao W et al. Estrogen Deficiency Induces Mitochondrial Damage Prior to Emergence of Cognitive Deficits in a Postmenopausal Mouse Model. Front Aging Neurosci 13:713819 (2021). PubMed: 34335235
Zhang X et al. Trib1 deficiency causes brown adipose respiratory chain depletion and mitochondrial disorder. Cell Death Dis 12:1098 (2021). PubMed: 34811364
Bertan F et al. Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution. Cell Rep Methods 1:100002 (2021). PubMed: 35474694
Zhang Q et al. Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. Nucleic Acids Res 49:4689-4704 (2021). PubMed: 33836087

View all Publications for this product

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Application

Western blot

Sample

Mouse Tissue lysate - other (Isolated mitochondria)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

10 µg

Specification

Isolated mitochondria

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Nov 29 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (16)

Customer reviews and Q&As

Western blot abreview for Anti-UQCRC2 antibody [EPR13051]