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RecombinantRabMAb

Recombinant Anti-UQCRC2 antibody [EPR13051] (ab203832)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (13)

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Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Flow Cytometry (Intracellular) - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)
  • Anti-UQCRC2 antibody [EPR13051] (ab203832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13051] to UQCRC2
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

You may also be interested in

Protein
Product image
Recombinant Human UQCRC2 protein (denatured) (ab134532)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-SDHC antibody [EPR11035(B)] (ab155999)

View more associated products

Overview

  • Product name

    Anti-UQCRC2 antibody [EPR13051]
    See all UQCRC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13051] to UQCRC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain, fetal heart and fetal kidney lysates; HepG2, HEK-293, Jurkat, C6 and PC-12 cell lysates; Mouse heart, rat brain and rat kidney lysates. IHC-P: Human liver tissue. ICC/IF: HepG2 and Jurkat cells. Flow Cyt (intra): Jurkat cells. IP: HepG2 and HEK-293 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13051
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex III

Associated products

  • Alternative Versions

    • Anti-UQCRC2 antibody [EPR13051] - BSA and Azide free (ab240368)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human UQCRC2 protein (denatured) (ab134532)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF
1/300.
IP
1/50.
Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF
1/300.
IP
1/50.

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similarities

    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P22695 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7385 Human
    • Entrez Gene: 67003 Mouse
    • Entrez Gene: 293448 Rat
    • Omim: 191329 Human
    • SwissProt: P22695 Human
    • SwissProt: Q9DB77 Mouse
    • SwissProt: P32551 Rat
    • Unigene: 528803 Human
    • Unigene: 334206 Mouse
    • Unigene: 2334 Rat
    see all
  • Alternative names

    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Images

  • Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    All lanes : Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : C6 (Rat glial tumor cells) cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRC2 with ab203832 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Flow Cytometry (Intracellular) - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    UQCRC2 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
    Lane 1: HepG2 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

    UQCRC2 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab203832 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203832 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-UQCRC2 antibody [EPR13051] (ab203832)
    Anti-UQCRC2 antibody [EPR13051] (ab203832)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (13)

Publishing research using ab203832? Please let us know so that we can cite the reference in this datasheet.

ab203832 has been referenced in 13 publications.

  • Zhang Q  et al. Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. Nucleic Acids Res 49:4689-4704 (2021). PubMed: 33836087
  • Zhao W  et al. Estrogen Deficiency Induces Mitochondrial Damage Prior to Emergence of Cognitive Deficits in a Postmenopausal Mouse Model. Front Aging Neurosci 13:713819 (2021). PubMed: 34335235
  • Su F  et al. Quantitative proteomics identified 3 oxidative phosphorylation genes with clinical prognostic significance in gastric cancer. J Cell Mol Med 24:10842-10854 (2020). PubMed: 32757436
  • Li K  et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
  • Li D  et al. Variation in energy metabolism arising from the effect of the tumor microenvironment on cell biological behaviors of bladder cancer cells and endothelial cells. Biofactors 46:64-75 (2020). PubMed: 31580525
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-UQCRC2 antibody [EPR13051]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Isolated mitochondria)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
Isolated mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Nov 29 2017

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