Anti-UQCRFS1/RISP antibody [5A5] (ab14746)
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)](https://www.abcam.com/ps/products/14/ab14746/Images/ab14746-22106-anti-uqcrfs1-risp-antibody-5a5-western-blot.jpg "Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)")
Key features and details
- Mouse monoclonal [5A5] to UQCRFS1/RISP
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-UQCRFS1/RISP antibody [5A5]
See all UQCRFS1/RISP primary antibodies -
Description
Mouse monoclonal [5A5] to UQCRFS1/RISP -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Pig
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Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, bovine, rat and murine heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in Raji cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
5A5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-P | (1) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
Sequence similarities
Contains 1 Rieske domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
Alternative names
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
Images
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Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 0.5 µg/ml
Lane 1 : Hela Whole Cell Lysate - Hydroxyurea Treated
Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated
Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)](https://www.abcam.com/ps/products/14/ab14746/Images/ab14746-22105-anti-uqcrfs1-risp-antibody-5a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)](https://www.abcam.com/ps/products/14/ab14746/Images/ab14746-22104-anti-uqcrfs1-risp-antibody-5a5-western-blot.jpg)
Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 1 µg/ml
Lane 1 : Isolated mitochondria from human heart at 5 µg
Lane 2 : Isolated mitochondria from bovine heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg -
![Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)](https://www.abcam.com/ps/products/14/ab14746/Images/ab14746-22102-anti-uqcrfs1-risp-antibody-5a5-flow-cytometry.jpg)
Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (73)
ab14746 has been referenced in 73 publications.
- Acoba MG et al. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep 34:108869 (2021). PubMed: 33730581
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309
- Lee CQE et al. Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. Nat Commun 12:2130 (2021). PubMed: 33837217
- Weber RA et al. Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation. Mol Cell 77:645-655.e7 (2020). PubMed: 31983508
- Gusic M et al. Bi-Allelic UQCRFS1 Variants Are Associated with Mitochondrial Complex III Deficiency, Cardiomyopathy, and Alopecia Totalis. Am J Hum Genet 106:102-111 (2020). PubMed: 31883641