Recombinant Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
![Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228193-ab191079b.jpg "Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal
See all UQCRFS1/RISP primary antibodies -
Description
Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Raji, 293, PC12, mouse brain and Human fetal heart lysates; Human kidney tissue; HepG2 and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16289 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/280.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
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| IP |
1/90.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/280. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
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IP
1/90. |
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WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
Sequence similarities
Contains 1 Rieske domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
Alternative names
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
Images
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Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lanes 2-3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa -
![Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228198-ab191079f.jpg)
Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Flow Cytometry (Intracellular) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228197-ab191079e.jpg)
Flow Cytometry (Intracellular) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1/RISP with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228195-ab191079d.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228194-ab191079c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-228192-ab191079.jpg)
Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa -
![Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)](https://www.abcam.com/ps/products/191/ab191079/Images/ab191079-7-benefits-of-recombinant-antibodies.png)
Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab191079 has been referenced in 1 publication.
- Chen XF et al. Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats. Nutr Diabetes 8:1 (2018). PubMed: 29330446