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    products/primary-antibodies/usp11-antibody-epr4346-ab109232.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-USP11 antibody [EPR4346] (ab109232)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (11)

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Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
  • Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)
  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
  • Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)
  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
  • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
  • Anti-USP11 antibody [EPR4346] (ab109232)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4346] to USP11
  • Suitable for: WB, IP, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-USP11 antibody [EPR4346]
    See all USP11 primary antibodies
  • Description

    Rabbit monoclonal [EPR4346] to USP11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, Jurkat, Human testis, Human fetal kidney and LnCaP cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4346
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Alternative Versions

    • Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • LNCaP nuclear extract lysate (ab14857)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP
1/10 - 1/300.
Flow Cyt (Intra)
1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP
1/10 - 1/300.
Flow Cyt (Intra)
1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 DUSP domain.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. Associates with chromatin.
  • Target information above from: UniProt accession P51784 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8237 Human
    • Entrez Gene: 236733 Mouse
    • Entrez Gene: 408217 Rat
    • Omim: 300050 Human
    • SwissProt: P51784 Human
    • SwissProt: Q99K46 Mouse
    • Unigene: 171501 Human
    • Unigene: 34489 Mouse
    • Alternative names

      • Deubiquitinating enzyme 11 antibody
      • Ubiquitin carboxyl-terminal hydrolase 11 antibody
      • Ubiquitin carboxyl-terminal hydrolase X linked antibody
      • ubiquitin specific peptidase 11 antibody
      • Ubiquitin specific protease 11 antibody
      • Ubiquitin thiolesterase 11 antibody
      • Ubiquitin-specific-processing protease 11 antibody
      • UBP11_HUMAN antibody
      • UHX1 antibody
      • USP11 antibody
      see all

    Images

    • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP11 knockout HAP1 cell lysate (20 µg)
      Lane 3: LNCaP cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109232 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109232 was shown to specifically react with USP11 when USP11 knockout samples were used. Wild-type and USP11 knockout samples were subjected to SDS-PAGE. ab109232 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)
      Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)

      Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab109232 at a dilution of 1 in 950 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

       

    • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
      Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
      ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1000 µg

      Predicted band size: 110 kDa
      Observed band size: 110 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)
      Flow Cytometry (Intracellular) - Anti-USP11 antibody [EPR4346] (ab109232)

      Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling USP11 with unpurified ab109232 at 1/570 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line). 

       

       

    • Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
      Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)
      ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)

      Lane 1 : HEK293 lysate at 20 µg
      Lane 2 : human fetal kidney lysate

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (purified)

      Lane 1 : Jurkat lysate
      Lane 2 : human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      Western blot - Anti-USP11 antibody [EPR4346] (ab109232)
      All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human testis cell lysate
      Lane 4 : Huma fetal kidney cell lysate
      Lane 5 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa

    • Anti-USP11 antibody [EPR4346] (ab109232)
      Anti-USP11 antibody [EPR4346] (ab109232)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab109232? Please let us know so that we can cite the reference in this datasheet.

    ab109232 has been referenced in 11 publications.

    • Zhang W  et al. Circ_DOCK1 regulates USP11 through miR-132-3p to control colorectal cancer progression. World J Surg Oncol 19:67 (2021). PubMed: 33685455
    • Zhang X  et al. A pro-inflammatory mediator USP11 enhances the stability of p53 and inhibits KLF2 in intracerebral hemorrhage. Mol Ther Methods Clin Dev 21:681-692 (2021). PubMed: 34141823
    • Ramirez J  et al. A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes. Int J Mol Sci 22:N/A (2021). PubMed: 34063716
    • Park MK  et al. PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression. Nat Commun 10:636 (2019). PubMed: 30733438
    • Wang W  et al. Upregulation of USP11 promotes epithelial-to-mesenchymal transition by deubiquitinating Snail in ovarian cancer. Oncol Rep 41:1739-1748 (2019). PubMed: 30569152
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-USP11 antibody [EPR4346]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 20 2020

    Western blot abreview for Anti-USP11 antibody [EPR4346]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    colon cancer
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 13 2020

    Flow Cytometry abreview for Anti-USP11 antibody [EPR4346]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    ebioscience Fixation/Permeablization kit
    Permeabilization
    Yes - ebioscience Fixation/Permeablization kit
    Sample
    Mouse Cell (Lymphocytes from peripheral lymph nodes and spleen)
    Specification
    Lymphocytes from peripheral lymph nodes and spleen
    Gating Strategy
    Lymphocytes were selected using FSC and SSC followed by gating on viable cells.
    Preparation
    Cell harvesting/tissue preparation method: Extracting spleen and lymph nodes during necropsy, followed by mashing through 70 micron filter
    Sample buffer: Sample collected in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Roman Istomine

    Verified customer

    Submitted Oct 22 2014

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