Anti-USP15 antibody (ab71713)
Key features and details
- Rabbit polyclonal to USP15
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-USP15 antibody
See all USP15 primary antibodies -
Description
Rabbit polyclonal to USP15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human USP15.
Database link: Q9Y4E8 -
Positive control
- WB: HeLa, K-562, MCF-7, U2OS, RKO, TCMK-1, and NIH/3T3 cells. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 112 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 112 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. -
Tissue specificity
Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 DUSP domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome. - Information by UniProt
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Database links
- Entrez Gene: 395644 Chicken
- Entrez Gene: 538284 Cow
- Entrez Gene: 100055857 Horse
- Entrez Gene: 9958 Human
- Entrez Gene: 14479 Mouse
- Entrez Gene: 100356438 Rabbit
- Entrez Gene: 171329 Rat
- Entrez Gene: 100542201 Turkey
see all -
Alternative names
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
see all
Images
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Western blot - Anti-USP15 antibody (ab71713)All lanes : Anti-USP15 antibody (ab71713) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : K-562 whole cell lysate at 50 µg
Lane 3 : MCF-7 whole cell lysate at 50 µg
Lane 4 : U2OS whole cell lysate at 50 µg
Lane 5 : RKO whole cell lysate at 50 µg
Lane 6 : TCMK-1 whole cell lysate at 50 µg
Lane 7 : NIH/3T3 whole cell lysate
Predicted band size: 112 kDa -
Immunoprecipitation - Anti-USP15 antibody (ab71713)USP15 was immunoprecipitated from HeLa whole cell lysate with ab71713 at 6 µg (1.0 mg per IP reaction; 20% of IP loaded).
Lane 1: ab71713
Lane 2: ab71713 at 6 µg per reaction.
Lane 3: Control IgG
Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab71713 has been referenced in 8 publications.
- Yang H et al. GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. iScience 25:104952 (2022). PubMed: 36065190
- Ling J et al. A Regulatory Network Analysis of the Importance of USP15 in Breast Cancer Metastasis and Prognosis. J Oncol 2022:1427726 (2022). PubMed: 36213818
- Wang W et al. Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-ß/SMAD2 signaling. Arthritis Res Ther 23:84 (2021). PubMed: 33726807
- Chen LL et al. USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2. Sci Adv 6:N/A (2020). PubMed: 32948596
- van den Berk P et al. USP15 Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells. Cell Rep 33:108533 (2020). PubMed: 33378683
- Niederkorn M et al. TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis. Cell Rep 30:2776-2790.e6 (2020). PubMed: 32101751
- Fang L et al. MYEOV functions as an amplified competing endogenous RNA in promoting metastasis by activating TGF-ß pathway in NSCLC. Oncogene 38:896-912 (2019). PubMed: 30181549
- Zhou L et al. USP15 inhibits multiple myeloma cell apoptosis through activating a feedback loop with the transcription factor NF-?Bp65. Exp Mol Med 50:151 (2018). PubMed: 30459344