Recombinant Anti-USP22 antibody [EPR4352(2)] (ab109435)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4352(2)] to USP22
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-USP22 antibody [EPR4352(2)]
See all USP22 primary antibodies -
Description
Rabbit monoclonal [EPR4352(2)] to USP22 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HAP1, human placenta and HT-1376 lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 1.13 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4352(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 60 kDa. |
Target
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Function
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. -
Tissue specificity
Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver. -
Sequence similarities
Belongs to the peptidase C19 family. UBP8 subfamily.
Contains 1 UBP-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23326 Human
- Omim: 612116 Human
- SwissProt: Q9UPT9 Human
- Unigene: 462492 Human
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Alternative names
- Deubiquitinating enzyme 22 antibody
- KIAA1063 antibody
- Ubiquitin carboxyl terminal hydrolase 22 antibody
see all
Images
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All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : USP22 knockout HeLa cell lysate
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-USP22 antibody [EPR4352(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109435 was shown to bind specifically to USP22. A band was observed at 59 kDa in wild-type HeLa cell lysates with no signal observed at this size in usp22 knockout cell line ab264888 (knockout cell lysate ab257789). To generate this image, wild-type and usp22 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP22 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109435 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
Ab109435 was shown to specifically react with USP22 in wild-type cells along with additional cross-reactive bands. The band was not seen in USP22 knockout HAP1 cells. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. ab109435 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human placenta lysate
Lane 3 : HT-1376 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells using 1/10 ab109435 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109435 has been referenced in 1 publication.
- Liu Z et al. USP22 regulates the formation and function of placental vasculature during the development of fetal growth restriction. Placenta 111:19-25 (2021). PubMed: 34130183