Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Uteroglobin antibody [EPR19846] (ab213203)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)
  • Anti-Uteroglobin antibody [EPR19846] (ab213203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19846] to Uteroglobin
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Uteroglobin antibody [EPR19846]
    See all Uteroglobin primary antibodies

  • Description

    Rabbit monoclonal [EPR19846] to Uteroglobin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse and rat lung tissues. IP: Mouse lung lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19846

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).

We don't recommend this antibody for rat in WB. In our hands rat tissues showed negative staining.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

In IHC, under our experimental conditions, this antibody showed specific staining in bronchial epithelium cells of both rat and mouse; however, there was non-specific staining on the pulmonary alveoli of mouse lung. Our collaborator observed the correct staining on the airway epithelium of mouse lung using immunofluorescence detection method.
IHC-Fr
1/500.

(Use 1/100 for rat tissue).

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
1/30.

We don't recommend this antibody for rat in IP.  In our hands rat tissues showed negative staining.
Notes
WB
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).

We don't recommend this antibody for rat in WB. In our hands rat tissues showed negative staining.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

In IHC, under our experimental conditions, this antibody showed specific staining in bronchial epithelium cells of both rat and mouse; however, there was non-specific staining on the pulmonary alveoli of mouse lung. Our collaborator observed the correct staining on the airway epithelium of mouse lung using immunofluorescence detection method.
IHC-Fr
1/500.

(Use 1/100 for rat tissue).

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
1/30.

We don't recommend this antibody for rat in IP.  In our hands rat tissues showed negative staining.
Application notes
Is unsuitable for ICC.

Target

  • Function

    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

  • Tissue specificity

    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).

  • Sequence similarities

    Belongs to the secretoglobin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Blastokinin antibody
      • CC10 antibody
      • CC16 antibody
      • CCPBP antibody
      • CCSP antibody
      • Clara cell phospholipid binding protein antibody
      • Clara cell phospholipid-binding protein antibody
      • Clara cell specific 10 kD protein antibody
      • Clara cells 10 kDa secretory protein antibody
      • OTTHUMP00000236107 antibody
      • SCGB1A1 antibody
      • Secretoglobin family 1A member 1 antibody
      • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
      • UG antibody
      • UGB antibody
      • UP-1 antibody
      • UP1 antibody
      • Urinary protein 1 antibody
      • Urine protein 1 antibody
      • UTER_HUMAN antibody
      • Uteroglobin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

      Strong cytoplasmic staining on the airway epithelium of rat lung is observed [PMID: 22916196].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

      Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

      The nuclear counterstain is DAPI (blue).

      Heat mediated antigen retrieval using citrate buffer pH 6.0

      The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    • Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      All lanes : Anti-Uteroglobin antibody [EPR19846] (ab213203) at 1/1000 dilution

      Lane 1 : Mouse lung lysate at 20 µg
      Lane 2 : Mouse heart lysate at 30 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 10 kDa
      Observed band size: 10 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Positive staining on the airway epithelium of mouse lung [PMID: 22916196].

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Positive staining on the airway epithelium of rat lung [PMID: 22916196].

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)

      Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

      Lane 1: Mouse lung lysate 10ug (Input).

      Lane 2: ab213203 IP in mouse lung lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-Uteroglobin antibody [EPR19846] (ab213203)
      Anti-Uteroglobin antibody [EPR19846] (ab213203)

    References (2)

    Publishing research using ab213203? Please let us know so that we can cite the reference in this datasheet.

    ab213203 has been referenced in 2 publications.

    • Almuntashiri S  et al. CC16 Regulates Inflammation, ROS Generation and Apoptosis in Bronchial Epithelial Cells during Klebsiella pneumoniae Infection. Int J Mol Sci 22:N/A (2021). PubMed: 34768890
    • Yao E  et al. Notch Signaling Controls Transdifferentiation of Pulmonary Neuroendocrine Cells in Response to Lung Injury. Stem Cells 36:377-391 (2018). PubMed: 29148109

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab213203.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com