Recombinant Anti-V2R antibody [EPR24555-59] (ab273021)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-1-anti-v2r-antibody-epr2455559-immunohistochemistry-human-kidney.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24555-59] to V2R
- Suitable for: IHC-P, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-V2R antibody [EPR24555-59]
See all V2R primary antibodies -
Description
Rabbit monoclonal [EPR24555-59] to V2R -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human kidney tissues. Dot Blot: Vasopressin V2 receptor peptide 2.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24555-59 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Dot blot |
1/1000.
|
| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Dot blot
1/1000. |
Target
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Function
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Kidney. -
Involvement in disease
Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis.
Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI) [MIM:304800]; also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 554 Human
- Omim: 300538 Human
- SwissProt: P30518 Human
- Unigene: 567240 Human
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Alternative names
- ADHR antibody
- Antidiuretic hormone receptor antibody
- Arginine vasopressin receptor 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling V2R with ab273021 at 1/100 (6.32 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on connecting ducts of human renal cortex. The section was incubated with ab273021 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-2-anti-v2r-antibody-epr2455559-immunohistochemistry-human-kidney1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling V2R with ab273021 at 1/100 (6.32 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on connecting ducts of human renal medulla. The section was incubated with ab273021 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-3-anti-v2r-antibody-epr2455559-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling V2R with ab273021 at 1/100 (6.32 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human liver. The section was incubated with ab273021 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-4-anti-v2r-antibody-epr2455559-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody [EPR24555-59] (ab273021)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling V2R with ab273021 at 1/100 (6.32 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum. The section was incubated with ab273021 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Dot Blot - Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-5-anti-v2r-antibody-epr2455559-dotblot-vasopressin-v2-receptor-peptide-1.jpg)
Dot Blot - Anti-V2R antibody [EPR24555-59] (ab273021)Dot blot analysis of V2R using ab273021 at 1:1000 (0.632 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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![Anti-V2R antibody [EPR24555-59] (ab273021)](https://www.abcam.com/ps/products/273/ab273021/Images/ab273021-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-V2R antibody [EPR24555-59] (ab273021)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab273021 has not yet been referenced specifically in any publications.