Recombinant Anti-Vasopressin antibody [EPR20602] (ab213708)
![Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288742-anti-vasopressin-antibody-epr20602-western-blot.jpg "Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20602] to Vasopressin
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Vasopressin antibody [EPR20602]
See all Vasopressin primary antibodies -
Description
Rabbit monoclonal [EPR20602] to Vasopressin -
Host species
Rabbit -
Specificity
In WB, FC and IP we detected Vasopressin only in overexpressing cells. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain paraventricular nucleus. Flow Cyt (intra): HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®. IP: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20602 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| WB |
1/2000. Predicted molecular weight: 17 kDa.
|
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| IP |
1/30.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IHC-Fr |
1/500.
Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0). |
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/2000. Predicted molecular weight: 17 kDa. |
|
IP
1/30. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/500. Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0). |
Target
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Relevance
Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 551 Human
- Entrez Gene: 11998 Mouse
- Entrez Gene: 24221 Rat
- Omim: 192340 Human
- SwissProt: P01185 Human
- SwissProt: P35455 Mouse
- SwissProt: P01186 Rat
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Alternative names
- ADH antibody
- Antidiuretic hormone antibody
- Arginine vasopressin neurophysin II antibody
see all
Images
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Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)All lanes : Anti-Vasopressin antibody [EPR20602] (ab213708) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288741-anti-vasopressin-antibody-epr20602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288740-anti-vasopressin-antibody-epr20602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288739-anti-vasopressin-antibody-epr20602-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288738-anti-vasopressin-antibody-epr20602-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288737-anti-vasopressin-antibody-epr20602-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected withVasopressin expression vector containing a myc-His-tag® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Co-stained with Alexa Fluor® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.
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![Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-288735-anti-vasopressin-antibody-epr20602-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate 10 µg (Input).
Lane 2: ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Vasopressin antibody [EPR20602] (ab213708)](https://www.abcam.com/ps/products/213/ab213708/Images/ab213708-8-benefits-of-recombinant-antibodies.png)
Anti-Vasopressin antibody [EPR20602] (ab213708)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab213708 has been referenced in 4 publications.
- Yaghmaeian Salmani B et al. Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes. Development 149:N/A (2022). PubMed: 35245348
- Crane-Smith Z et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
- Zhou L et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
- Ran X et al. Distribution of D1 and D2 receptor- immunoreactive neurons in the paraventricular nucleus of the hypothalamus in the rat. J Chem Neuroanat 98:97-103 (2019). PubMed: 31018158