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RecombinantRabMAb

Recombinant Anti-Vasopressin antibody [EPR20602] (ab213708)

Submit a review Submit a question References (3)
Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)
  • Anti-Vasopressin antibody [EPR20602] (ab213708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20602] to Vasopressin
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

Overview

  • Product name

    Anti-Vasopressin antibody [EPR20602]
    See all Vasopressin primary antibodies

  • Description

    Rabbit monoclonal [EPR20602] to Vasopressin

  • Host species

    Rabbit

  • Specificity

    In WB, FC and IP we detected Vasopressin only in overexpressing cells.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain paraventricular nucleus. Flow Cyt (intra): HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®. IP: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/2000. Predicted molecular weight: 17 kDa.
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0).
Notes
Flow Cyt (Intra)
1/500.
WB
1/2000. Predicted molecular weight: 17 kDa.
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0).

Target

  • Relevance

    Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • ADH antibody
      • Antidiuretic hormone antibody
      • Arginine vasopressin neurophysin II antibody
      • ARVP antibody
      • AVP antibody
      • AVP NPII antibody
      • copeptin antibody
      • Vasopressin neurophysin II copeptin antibody
      • VP antibody
      see all

    Images

    • Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)
      All lanes : Anti-Vasopressin antibody [EPR20602] (ab213708) at 1/2000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected withVasopressin expression vector containing a myc-His-tag® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

      Co-stained with Alexa Fluor® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining. 

       

       

    • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)
      Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)

      Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

      Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate 10 µg (Input). 

      Lane 2: ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-Vasopressin antibody [EPR20602] (ab213708)
      Anti-Vasopressin antibody [EPR20602] (ab213708)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab213708? Please let us know so that we can cite the reference in this datasheet.

    ab213708 has been referenced in 3 publications.

    • Crane-Smith Z  et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
    • Zhou L  et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
    • Ran X  et al. Distribution of D1 and D2 receptor- immunoreactive neurons in the paraventricular nucleus of the hypothalamus in the rat. J Chem Neuroanat 98:97-103 (2019). PubMed: 31018158

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