Recombinant Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
![Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-402242-anti-vav-proteins-antibody-ep482y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP482Y] to Vav proteins - BSA and Azide free
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Vav proteins antibody [EP482Y] - BSA and Azide free
See all Vav proteins primary antibodies -
Description
Rabbit monoclonal [EP482Y] to Vav proteins - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247298 is the carrier-free version of ab40875.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP482Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
Also 101 or 87 kDa, depending on the isoform.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Relevance
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
Cellular localization
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3 -
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 7409 Human
- Entrez Gene: 7410 Human
- Omim: 164875 Human
- Omim: 600428 Human
- Omim: 605541 Human
- SwissProt: P15498 Human
- SwissProt: P52735 Human
see all -
Alternative names
- 2810040F13Rik antibody
- A530094I06Rik antibody
- AA986410 antibody
see all
Images
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Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
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![Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-1-anti-vav-proteins-antibody-ep482y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)This data was developed using ab40875, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-3-benefits-of-recombinant-antibodies.png)
Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247298 has not yet been referenced specifically in any publications.
