Recombinant Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)
![Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)](https://www.abcam.com/ps/products/76/ab76225/Images/ab76225-300460-ab76225WB1.jpg "Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174)
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-VAV1 (phospho Y174) antibody [EP510Y]
See all VAV1 primary antibodies -
Description
Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, Dot blotmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VAV1 (phospho Y174). The exact sequence is proprietary.
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Positive control
- WB: Jurkat cell lysate treated with pervanadate. ICC/IF: Jurkat cell lysate treated with pervanadate. Flow Cyt (intra): WB: Jurkat cell lysate treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP510Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
|
| ICC/IF |
1/100 - 1/500.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
|
ICC/IF
1/100 - 1/500. |
|
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
Tissue specificity
Widely expressed in hematopoietic cells but not in other cell types. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The DH domain is involved in interaction with CCPG1. -
Post-translational
modificationsPhosphorylated on tyrosine residues. - Information by UniProt
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Database links
- Entrez Gene: 7409 Human
- Omim: 164875 Human
- SwissProt: P15498 Human
- Unigene: 116237 Human
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Alternative names
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
Images
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Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)All lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 101 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)](https://www.abcam.com/ps/products/76/ab76225/Images/ab76225-265971-ab76225-jurkat-treated.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)Immunocytochemistry/Immunofluorescence analysis of Untreated Jurkat cells and Pervanadate (1mM,30min) treated Jurkat cells labelling VAV1 (phospho Y174) with purified ab76225 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1:1000 (1ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody(ab150120)
Negative Control 2: Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)
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![Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)](https://www.abcam.com/ps/products/76/ab76225/Images/ab76225-276003-ab76225FC1.jpg)
Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).
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![Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)](https://www.abcam.com/ps/products/76/ab76225/Images/ab76225-255471-ab76225Dot.jpg)
Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)](https://www.abcam.com/ps/products/76/ab76225/Images/ab76225-6-benefits-of-recombinant-antibodies.png)
Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab76225 has been referenced in 14 publications.
- Raiter A et al. A novel role for an old target: CD45 for breast cancer immunotherapy. Oncoimmunology 10:1929725 (2021). PubMed: 34104545
- Guedan S et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest 130:3087-3097 (2020). PubMed: 32069268
- Zhang C et al. CD100-plexin-B1 induces epithelial-mesenchymal transition of head and neck squamous cell carcinoma and promotes metastasis. Cancer Lett 455:1-13 (2019). PubMed: 30981760
- Zhang Z et al. CEACAM1 regulates the IL-6 mediated fever response to LPS through the RP105 receptor in murine monocytes. BMC Immunol 20:7 (2019). PubMed: 30674283
- Li Y et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067