Recombinant Anti-VCAM1 antibody [EPR5038(2)] (ab174279)
![Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-464295-anti-vcam1-antibody-epr50382-anti-vcam1-antibody-epr50382-bsa-and-azide-free-western-blot-wildtype-a549.jpg "Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5038(2)] to VCAM1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-VCAM1 antibody [EPR5038(2)]
See all VCAM1 primary antibodies -
Description
Rabbit monoclonal [EPR5038(2)] to VCAM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, HuT-78, NIH3T3, HUVEC, bEND.3, Hut-78 and LADMAC lysates; Wild-type A549 TNF-a treated (10 ng/mL, 16h) cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5038(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls. |
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa). Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls. |
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IP
1/10 - 1/100. |
Target
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7412 Human
- Entrez Gene: 22329 Mouse
- Entrez Gene: 25361 Rat
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- SwissProt: P29533 Mouse
see all -
Alternative names
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
Images
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Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : Wild-type A549 TNF-a treated (10 ng/mL, 16h) cell lysate
Lane 3 : VCAM1 knockout A549 cell lysate
Lane 4 : VCAM1 knockout A549 TNF-a treated (10 ng/mL, 16h) cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsab174279 was shown to react with VCAM1 in treated wild-type A549 cells in western blot. Loss of signal was observed when treated VCAM1 knockout cell line ab273758 (knockout cell lysate ab275504) was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab174279 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1 in 5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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![Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-394774-AntiVCAM1-antibody-ab174279.jpg)
Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HUVEC treated with 10 ng/ml TNF-a for 16 h, whole cell lysate
Lane 3 : bEND.3 (Mouse brain endothelioma) whole cell lysate
Lane 4 : bEND.3 treated with 10 µg/ml LPS for 24 h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-394773-AntiVCAM1-antibody-ab174279.jpg)
Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution
Lane 1 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lane 5 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-199261-anti-vcam1-antibody-epr50382-western-blot.jpg)
Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : HuT-78 lysate
Lane 3 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 81 kDa -
![Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-199260-anti-vcam1-antibody-epr50382-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.
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![Anti-VCAM1 antibody [EPR5038(2)] (ab174279)](https://www.abcam.com/ps/products/174/ab174279/Images/ab174279-5-benefits-of-recombinant-antibodies.png)
Anti-VCAM1 antibody [EPR5038(2)] (ab174279)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab174279 has been referenced in 15 publications.
- Lin ZH et al. The effects of estradiol on inflammatory and endothelial dysfunction in rats with preeclampsia. Int J Mol Med 45:825-835 (2020). PubMed: 31985028
- Yang X et al. FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration. J Am Heart Assoc 9:e014333 (2020). PubMed: 32067586
- Yang CM et al. Mevastatin-Induced AP-1-Dependent HO-1 Expression Suppresses Vascular Cell Adhesion Molecule-1 Expression and Monocyte Adhesion on Human Pulmonary Alveolar Epithelial Cells Challenged with TNF-a. Biomolecules 10:N/A (2020). PubMed: 32121588
- Sun H et al. Long Non-Coding RNA (LncRNA)-ATB Promotes Inflammation, Cell Apoptosis and Senescence in Transforming Growth Factor-ß1 (TGF-ß1) Induced Human Kidney 2 (HK-2) Cells via TGFß/SMAD2/3 Signaling Pathway. Med Sci Monit 26:e922029 (2020). PubMed: 32447340
- Vassileff N et al. Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues. Cells 9:N/A (2020). PubMed: 32708779