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    products/primary-antibodies/vcam1-antibody-epr5047-ab134047.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Vascular
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-VCAM1 antibody [EPR5047] (ab134047)

  • Datasheet
  • SDS
Reviews (15)Q&A (3)References (198)

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Indirect ELISA - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Flow Cytometry (Intracellular) - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunoprecipitation - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
  • Anti-VCAM1 antibody [EPR5047] (ab134047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5047] to VCAM1
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF, Indirect ELISA
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free FITC HRP PE

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Overview

  • Product name

    Anti-VCAM1 antibody [EPR5047]
    See all VCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5047] to VCAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF, Indirect ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse VCAM1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P29533
    (Peptide available as ab156177)

  • Positive control

    • IHC-P: Human spleen and tonsil tissue; mouse spleen tissue. WB: Mouse kidney, brain and spleen tissue lysate; rat brain, spleen and kidney tissue lysate; human fetal liver tissue lysate; NIH/3T3, LADMAC, HuT-78, TNF-a treated HUVEC, and LPS treated bEnd.3 cell lysates; Wild-type A549 and HUVEC TNF-a treated (10 ng/mL, 16h) cell lysate Flow Cyt (intra): K562 cells. ICC/IF: HUVEC TNF-a treated (10 ng/mL, 16h); K562 cells. IP: Human fetal liver tissue lysate.
  • General notes

    Vascular cell adhesion protein 1 (VCAM) is a protein that is encoded by the VCAM1 gene in humans. It plays a role in functioning as a cell adhesion molecule and is thought to participate in monocyte recruitment to atherosclerotic sites, and as such is highly overexpressed in brain inflammation.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5047
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (ab194319)
    • HRP Anti-VCAM1 antibody [EPR5047] (ab195540)
    • Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
    • PE Anti-VCAM1 antibody [EPR5047] (ab223981)
    • FITC Anti-VCAM1 antibody [EPR5047] (ab223982)
    • APC Anti-VCAM1 antibody [EPR5047] (ab223983)
    • Anti-VCAM1 antibody [EPR5047] - BSA and Azide free (ab271899)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human VCAM1 knockout A549 cell line (ab273758)
  • KO cell lysates

    • Human VCAM1 knockout A549 cell lysate (ab275504)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

IP
1/40.
IHC-P (5)
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

Flow Cyt (Intra)
1/40.
ICC/IF (3)
1/250.
Indirect ELISA
Use at an assay dependent concentration.
Notes
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

IP
1/40.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

Flow Cyt (Intra)
1/40.
ICC/IF
1/250.
Indirect ELISA
Use at an assay dependent concentration.

Target

  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications

    Sialoglycoprotein.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P19320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7412 Human
    • Entrez Gene: 22329 Mouse
    • Entrez Gene: 25361 Rat
    • GenBank: NP_001069.1 Human
    • GenBank: NP_001186763.1 Human
    • Omim: 192225 Human
    • SwissProt: P19320 Human
    • SwissProt: P29533 Mouse
    • SwissProt: P29534 Rat
    • Unigene: 109225 Human
    • Unigene: 440909 Mouse
    • Unigene: 76649 Mouse
    • Unigene: 11267 Rat
    see all
  • Alternative names

    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Images

  • Indirect ELISA - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Indirect ELISA - Anti-VCAM1 antibody [EPR5047] (ab134047)

    ELISA using ab134047 at varying antibody concentrations and antigen concentration at 250 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/2000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : Wild-type A549 TNF-a treated (10 ng/mL, 16h) cell lysate
    Lane 3 : VCAM1 knockout A549 cell lysate
    Lane 4 : VCAM1 knockout A549 TNF-a treated (10 ng/mL, 16h) cell lysate
    Lane 5 : HUVEC cell lysate
    Lane 6 : HUVEC TNF-a treated (16 ng/mL, 16h) cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 81 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab134047 observed at 105 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab134047 was shown to react with VCAM1 in treated wild-type A549 cells in Western blot with loss of signal observed in treated VCAM1 knockout cell line ab273758 (knockout cell lysate ab275504). Wild-type A549 and VCAM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134047 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)

    ab134047 staining VCAM1 in HUVEC cells treated with TNF-α (ab55237) at 10 ng/ml for 16 hours. The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab134047 at 2μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 647) (ab150119) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/1000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell) treated with 10 ng/ml TNF-a for 16 h, whole cell lysate
    Lane 3 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 4 : bEnd.3 (Mouse brain endothelioma) treated with 10 µg/ml LPS for 24 h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 seconds


    Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

    Blocking/Diluting buffer: 5% NFDM/TBST.

    Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/1000 dilution

    Lane 1 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
    Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
    Lane 5 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 7 seconds


    Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab134047 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] (ab134047)

    Immunofluorescent staining of K562 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab134047 at a dilution of 1/250. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Flow Cytometry (Intracellular) - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Flow Cytometry (Intracellular) - Anti-VCAM1 antibody [EPR5047] (ab134047)

    Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling VCAM1 with purified ab134047 at 1/40 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

     

     

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified)

    Lane 1 : Mouse kidney
    Lane 2 : Rat spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunoprecipitation - Anti-VCAM1 antibody [EPR5047] (ab134047)

    VCAM1 was immunoprecipitated from Human fetal liver lysate with ab134047 at 1/110 dilution.

    Western blot was performed from the immunoprecipitate using ab134047 at 1/1000 dilution.

    VeriBlot for IP secondary antibody (Peroxidase conjugated),was used as secondary antibody at 1/1000 dilution.

    Lane 1: Human fetal liver lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)

    IHC image of VCAM1 staining in Human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab134047, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/2000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + HuT-78 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [EPR5047] (ab134047)

    IHC image of VCAM1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab134047, 1/200 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    Western blot - Anti-VCAM1 antibody [EPR5047] (ab134047)
    All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HuT 78 cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse spleen tissue lysate
    Lane 7 : Rat brain tissue lysate
    Lane 8 : Rat kidney tissue lysate
    Lane 9 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 81 kDa

  • Anti-VCAM1 antibody [EPR5047] (ab134047)
    Anti-VCAM1 antibody [EPR5047] (ab134047)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

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References (198)

Publishing research using ab134047? Please let us know so that we can cite the reference in this datasheet.

ab134047 has been referenced in 198 publications.

  • Yin D  et al. Long non-coding RNA RMST promotes oxygen-glucose deprivation-induced injury in brain microvascular endothelial cells by regulating miR-204-5p/VCAM1 axis. Life Sci N/A:119244 (2021). PubMed: 33607153
  • Huang J  et al. Role of tubular epithelial arginase-II in renal inflammaging. NPJ Aging Mech Dis 7:5 (2021). PubMed: 33654066
  • Liu Y  et al. Overexpression of Activating Transcription Factor 3 Alleviates Cardiac Microvascular Ischemia/Reperfusion Injury in Rats. Front Pharmacol 12:598959 (2021). PubMed: 33679395
  • Eidson LN  et al. Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction. Sci Rep 11:5533 (2021). PubMed: 33692398
  • Liu B  et al. Expression and significance of microRNA-126 and VCAM-1 in placental tissues of women with early-onset preeclampsia. J Obstet Gynaecol Res 47:2042-2050 (2021). PubMed: 33694224
View all Publications for this product

Customer reviews and Q&As

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1-10 of 18 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-VCAM1 antibody [EPR5047]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 03 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Soledad Camolotto

Verified customer

Submitted Mar 18 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (muscle stem cell)
Permeabilization
Yes - 0.5% tritonx-100
Specification
muscle stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 25 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VCAM1 antibody [EPR5047]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 10 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 10 2020

Western blot abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Choroid)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Choroid
Blocking step
Protein-Free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 25 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-VCAM1 antibody [EPR5047]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Frontal Cortex (Brain))
Permeabilization
Yes - 0.1% TritonX-100
Specification
Frontal Cortex (Brain)
Blocking step
Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 19 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Synovial pannus tissue, ankle joint)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Synovial pannus tissue, ankle joint
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Han-Byul Lee

Verified customer

Submitted May 28 2019

Immunohistochemistry (Frozen sections) abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor tissue)
Permeabilization
Yes - 0.3% triton X-100
Specification
tumor tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 04 2019

Immunocytochemistry abreview for Anti-VCAM1 antibody [EPR5047]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Pig Cultured Cells (Endothelial cell)
Permeabilization
Yes - Methanol
Specification
Endothelial cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 10 2018

1-10 of 18 Abreviews or Q&A

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