Anti-VCP antibody [3E8DC11] (ab110308)
![Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123580-anti-vcp-antibody-3e8dc11-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [3E8DC11] (ab110308)")
Key features and details
- Mouse monoclonal [3E8DC11] to VCP
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-VCP antibody [3E8DC11]
See all VCP primary antibodies -
Description
Mouse monoclonal [3E8DC11] to VCP -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human HDFn cells IP: Human, Rat, and Mouse liver samples; HepG2 cultured cell lysate Flow Cyt: HL-60 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The purity of ab110308 is near homogeneity, as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3E8DC11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 2 - 20 µg/ml.
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IP | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
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ICC/IF
Use a concentration of 2 - 20 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A. -
Involvement in disease
Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia. -
Sequence similarities
Belongs to the AAA ATPase family. -
Post-translational
modificationsPhosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. - Information by UniProt
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Database links
- Entrez Gene: 7415 Human
- Entrez Gene: 269523 Mouse
- Entrez Gene: 116643 Rat
- Omim: 601023 Human
- SwissProt: P55072 Human
- SwissProt: Q01853 Mouse
- SwissProt: P46462 Rat
- Unigene: 529782 Human
see all -
Alternative names
- 15S Mg(2+) ATPase p97 subunit antibody
- 15S Mg(2+)-ATPase p97 subunit antibody
- ALS14 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [3E8DC11] (ab110308)Immunocytochemistry image of ab110308-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110308 at 2 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in nucleus and cytoplasm.
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![Immunoprecipitation - Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123579-anti-vcp-antibody-3e8dc11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VCP antibody [3E8DC11] (ab110308)ab110308 pulls down the 89 kDa VCP protein in Human (lane 1), Rat (lane 2), and Mouse (lane 3) liver samples and Human HepG2 cultured cell lysate (lane 4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with sypro ruby gel stain. -
![Flow Cytometry - Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123578-anti-vcp-antibody-3e8dc11-flow-cytometry.jpg)
Flow Cytometry - Anti-VCP antibody [3E8DC11] (ab110308)HL-60 cells were stained with 1 µg/mL ab110308 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab110308 has been referenced in 1 publication.
- Zemoura K et al. Endoplasmic reticulum-associated degradation controls cell surface expression of ?-aminobutyric acid, type B receptors. J Biol Chem 288:34897-905 (2013). Other ; Human . PubMed: 24114844
