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    products/primary-antibodies/vdac1porin--vdac3-antibody-20b12af2-ab14734.pdf

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Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)

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  • SDS
Reviews (52)Q&A (25)References (426)

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Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)

Key features and details

  • Mouse monoclonal [20B12AF2] to VDAC1/Porin + VDAC3
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]
  • Description

    Mouse monoclonal [20B12AF2] to VDAC1/Porin + VDAC3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Goat, Cat, Dog, Pig, Drosophila melanogaster, Fish, Quail, Common marmoset, Dogfish, Catshark
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Isolated mitochondria from human, cow, rat and mouse heart. HepG2 cell lysate. ICC/IF: HeLa cells. Human fibroblasts. Flow Cyt: HepG2 cells.
  • General notes

    This antibody clone [20B12AF2] is manufactured by Abcam.

    If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituents: HEPES, Sodium chloride
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    20B12AF2
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Isotype/Loading Controls
    • Loading Controls
    • VDAC1 - Mitochondrial Preps
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • KO cell lines

    • Human VDAC1 (Porin) knockout HEK-293T cell line (ab255444)
  • KO cell lysates

    • Human VDAC1 (Porin) knockout HEK-293T cell lysate (ab263839)
  • Positive Controls

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (18)
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa.
ICC/IF (4)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Cellular localization

    VDAC1/Porin: Mitochondrion outer membrane. Cell membrane. VDAC3: Mitochondrial outer membrane.
  • Database links

    • Entrez Gene: 282119 Cow
    • Entrez Gene: 282716 Cow
    • Entrez Gene: 7416 Human
    • Entrez Gene: 7419 Human
    • Entrez Gene: 22333 Mouse
    • Entrez Gene: 22335 Mouse
    • Entrez Gene: 397010 Pig
    • Entrez Gene: 397651 Pig
    • Entrez Gene: 83529 Rat
    • Entrez Gene: 83532 Rat
    • Omim: 604492 Human
    • Omim: 610029 Human
    • SwissProt: P45879 Cow
    • SwissProt: Q9MZ13 Cow
    • SwissProt: P21796 Human
    • SwissProt: Q9Y277 Human
    • SwissProt: Q60931 Mouse
    • SwissProt: Q60932 Mouse
    • SwissProt: Q29380 Pig
    • SwissProt: Q9MZ16 Pig
    • SwissProt: Q9R1Z0 Rat
    • SwissProt: Q9Z2L0 Rat
    • Unigene: 519320 Human
    • Unigene: 709506 Human
    • Unigene: 3555 Mouse
    • Unigene: 54594 Rat
    see all

Images

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/1000 dilution

    Lane 1 : Wild-type Hap1 cell lysate
    Lane 2 : VDAC1 knockout Hap1 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : VDAC1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.


    Lanes 1 - 4: Merged signal (red and green). Green - ab14734 observed at 31 kDa. Red - loading control, ab181602 observed at 37 kDa. The lower band very close to VDAC1 is likely to be VDAC3.

    ab14734 was shown to react with VDAC1/Porin + VDAC3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255444 (knockout cell lysate ab263839) was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab14734 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)This image is courtesy of an Abreview by Michiel Krols.

    ab14734 staining VDAC1/Porin + VDAC3 in human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 3 minutes and blocked with 0.2% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in 0.5% BSA and 0.02% Triton X100 in PBS) for 16 hours at 4°C. An FITC-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/200.

    See Abreview

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/1000 dilution

    Lane 1 : N-GST tagged Recombinant Human VDAC1 (aa1 to 283) protein with NFDM/TBST
    Lane 2 : N-GST tagged Recombinant Human VDAC2 (aa 1 to 294) protein with NFDM/TBST
    Lane 3 : N-GST tagged Recombinant Human VDAC3 (aa 1 to 283) protein with NFDM/TBST

    Lysates/proteins at 0.01 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 33 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Diluting buffer: 5% NFDM /TBST

    N-GST tagged Recombinant Human VDAC1 protein is available as ab132481

    N-GST tagged Recombinant Human VDAC2 protein is available as ab152793

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/1000 dilution

    Lane 1 : Wild type HAP1 whole cell lysate with NFDM/TBST
    Lane 2 : VDAC1 knockout HAP1 whole cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 33 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Diluting buffer: 5% NFDM /TBST

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate with NFDM/TBST
    Lane 2 : HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate with NFDM/TBST
    Lane 3 : MEF (Mus musculus Embryo Fibroblast) whole cell lysate with NFDM/TBST
    Lane 4 : Rat brain tissue lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 33 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Diluting buffer: 5% NFDM /TBST

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)Sun Y et al Voltage-dependent anion channels (VDACs) recruit Parkin to defective mitochondria to promote mitochondrial autophagy. J Biol Chem. 2012 Nov 23;287(48):40652-60. doi: 10.1074/jbc.M112.419721.

    (A) The Anti-VDAC1/Porin + VDAC3 antibody recognizes VDAC1 and VDAC3 but not VDAC2.

    Lysates from wild-type MEFs and VDAC1/3-/- MEFs were analyzed by western blotting with Anti-VDAC1/Porin + VDAC3 (top panel) or anti Tom20 antibodies (bottom panel).

    (B) The Anti-VDAC1/Porin + VDAC3 antibody does not recognize VDAC2 in VDAC1/3-/- MEFs.

    HA-tagged VDAC1, 2 or 3 were expressed in VDAC1/3-/- MEFs, immunoprecipitated with anti-HA antibodies and analyzed by western blotting with anti-HA antibodies (top panel) or Anti-VDAC1/Porin + VDAC3 antibodies (bottom panel). The Anti-VDAC1/Porin + VDAC3 antibodies recognize HA-VDAC1 and HA-VDAC3, but not HA-VDAC2 . Note that HA-VDAC3 migrates at a lower molecular weight than HA-VDAC1 and HA-VDAC2 .

    Molecular weights are indicated in kDa.

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)Li et al PLoS One. 2016 Feb 22;11(2):e0149728. doi: 10.1371/journal.pone.0149728. eCollection 2016. Fig 5.

    Immunoblot analysis of endogenous VDAC1/Porin and VDAC3 in subcellular fractions of MA-10 cell lysates. VDAC1/Porin and VDAC3 are used as a mitochondrial marker proteins.

    Lane 1: Whole cell lysate

    Lane 2: Cytosolic fraction

    Lane 3: Mitochondrial-enriched fraction

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)

    Lane 1 : Isolated mitochondria from human heart at 15 µg
    Lane 2 : Isolated mitochondria from bovine heart at 6 µg
    Lane 3 : Isolated mitochondria from rat heart at 30 µg
    Lane 4 : Isolated mitochondria from mouse heart at 30 µg
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 30 µg

    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Extra bands in the mouse sample (lane 4) are due to the reaction of the secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)

    Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab14734 (red line).

    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14734, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

    This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)

    Immunofluoresence using ab14734 at 0.2 µg/ml on human fibroblasts (red).
    Nuclei were labeled with DAPI (blue).

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)Image courtesy of an anonymous abreview.
    Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3 minutes


    0.5% TBS-tween + Lait 5% NaN3 for 16 hours at 4ºC.

    See Abreview

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)
    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734)This image is courtesy of an anonymous Abreview
    All lanes : Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) at 1/5000 dilution

    Lanes 1-2 : Rat brain cell lysate (homogenate)
    Lanes 3-4 : Rat brain cell lysate (mitochondrial)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (426)

Publishing research using ab14734? Please let us know so that we can cite the reference in this datasheet.

ab14734 has been referenced in 426 publications.

  • Albuck AL  et al. Peroxynitrite decomposition catalyst enhances respiratory function in isolated brain mitochondria. Am J Physiol Heart Circ Physiol 320:H630-H641 (2021). PubMed: 33164581
  • Elswood J  et al. Autophagy regulates functional differentiation of mammary epithelial cells. Autophagy 17:420-438 (2021). PubMed: 31983267
  • Visuttijai K  et al. Progressive external ophthalmoplegia associated with novel MT-TN mutations. Acta Neurol Scand 143:103-108 (2021). PubMed: 32869280
  • Gibbs KW  et al. Aging Influences the Metabolic and Inflammatory Phenotype in an Experimental Mouse Model of Acute Lung Injury. J Gerontol A Biol Sci Med Sci 76:770-777 (2021). PubMed: 32997738
  • Goncalves RLS  et al. Cardiolipin deficiency in Barth syndrome is not associated with increased superoxide/H2 O2 production in heart and skeletal muscle mitochondria. FEBS Lett 595:415-432 (2021). PubMed: 33112430
View all Publications for this product

Customer reviews and Q&As

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1-10 of 77 Abreviews or Q&A

IHC - Wholemount abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Inconclusive
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (fat body)
Specification
fat body
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 09 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Appendix)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.2
Permeabilization
Yes - Tween-20
Specification
Appendix
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 16 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-VDAC1/Porin antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Huh7 cell line)
Permeabilization
Yes - 0.5% TX100
Specification
Human Huh7 cell line
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Apr 08 2022

Western blot abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human primary fibroblasts)
Gel Running Conditions
Reduced Denaturing (12% TGX)
Loading amount
30 µg
Specification
Human primary fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 02 2021

Western blot abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (Drosophila thorax)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Drosophila thorax
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2021

Western blot abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 13 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Aug 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 12 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 30 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 25 2020

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