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    products/primary-antibodies/vdac1porin-antibody-16g9e6bc4-mitochondrial-loading-control-ab110326.pdf

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Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (41)

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Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

    Key features and details

    • Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human VDAC1/Porin protein (ab132481)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Knockout
    Product image
    Human VDAC1 (Porin) knockout HEK-293T cell line (ab255444)

    View more associated products

    Overview

    • Product name

      Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
      See all VDAC1/Porin primary antibodies
    • Description

      Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Saccharomyces cerevisiae
    • Immunogen

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      ab110326 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      16G9E6BC4
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Isotype/Loading Controls
      • Loading Controls
      • VDAC1 - Mitochondrial Preps
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human VDAC1/Porin protein (ab132481)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110326 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

    Target

    • Function

      Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
    • Tissue specificity

      Heart, liver and skeletal muscle.
    • Sequence similarities

      Belongs to the eukaryotic mitochondrial porin family.
    • Domain

      Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
    • Cellular localization

      Mitochondrion outer membrane. Cell membrane.
    • Target information above from: UniProt accession P21796 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • SwissProt: P04840 Saccharomyces cerevisiae
      • Alternative names

        • N2441 antibody
        • OMP2 antibody
        • POR1 antibody
        • hVDAC1 antibody
        • MGC111064 antibody
        • Mitochondrial Porin antibody
        • Outer mitochondrial membrane protein porin 1 antibody
        • Plasmalemmal porin antibody
        • Porin 31HL antibody
        • Porin 31HM antibody
        • VDAC antibody
        • VDAC-1 antibody
        • Vdac1 antibody
        • VDAC1_HUMAN antibody
        • Voltage dependent anion channel 1 antibody
        • Voltage dependent anion selective channel protein 1 antibody
        • Voltage-dependent anion-selective channel protein 1 antibody
        • YNL055C antibody
        • YNL2441C antibody
        see all

      Images

      • Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.

        Predicted band size: 30 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (41)

      Publishing research using ab110326? Please let us know so that we can cite the reference in this datasheet.

      ab110326 has been referenced in 41 publications.

      • Brettrager EJ  et al. Sulfotransferase 4A1 activity facilitates sulfate-dependent cellular protection to oxidative stress. Sci Rep 12:1625 (2022). PubMed: 35102205
      • Liu Y  et al. Inokosterone from Gentiana rigescens Franch Extends the Longevity of Yeast and Mammalian Cells via Antioxidative Stress and Mitophagy Induction. Antioxidants (Basel) 11:N/A (2022). PubMed: 35204097
      • Nývltová E  et al. Coordination of metal center biogenesis in human cytochrome c oxidase. Nat Commun 13:3615 (2022). PubMed: 35750769
      • Kemmerer ZA  et al. UbiB proteins regulate cellular CoQ distribution in Saccharomyces cerevisiae. Nat Commun 12:4769 (2021). PubMed: 34362905
      • Broeskamp F  et al. Porin 1 Modulates Autophagy in Yeast. Cells 10:N/A (2021). PubMed: 34572064
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Candida albicans Cell lysate - other (Cytosolic and mitochondrial fractions)
      Gel Running Conditions
      Reduced Denaturing (4-12% precast gel)
      Loading amount
      15 µg
      Treatment
      Induced with 10 mM proline for 1 h
      Specification
      Cytosolic and mitochondrial fractions
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Fitz Gerald S. Silao

      Verified customer

      Submitted Apr 26 2021

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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