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    products/primary-antibodies/vdac1porin-antibody-16g9e6bc4-mitochondrial-loading-control-ab110326.pdf

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Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (33)

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Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

    Key features and details

    • Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human VDAC1/Porin protein (ab132481)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Knockout
    Product image
    Human VDAC1 (Porin) knockout HEK-293T cell line (ab255444)

    View more associated products

    Overview

    • Product name

      Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
      See all VDAC1/Porin primary antibodies
    • Description

      Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Saccharomyces cerevisiae
    • Immunogen

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      ab110326 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      16G9E6BC4
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Isotype/Loading Controls
      • Loading Controls
      • VDAC1 - Mitochondrial Preps
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human VDAC1/Porin protein (ab132481)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110326 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

    Target

    • Function

      Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
    • Tissue specificity

      Heart, liver and skeletal muscle.
    • Sequence similarities

      Belongs to the eukaryotic mitochondrial porin family.
    • Domain

      Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
    • Cellular localization

      Mitochondrion outer membrane. Cell membrane.
    • Target information above from: UniProt accession P21796 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • SwissProt: P04840 Saccharomyces cerevisiae
      • Alternative names

        • N2441 antibody
        • OMP2 antibody
        • POR1 antibody
        • hVDAC1 antibody
        • MGC111064 antibody
        • Mitochondrial Porin antibody
        • Outer mitochondrial membrane protein porin 1 antibody
        • Plasmalemmal porin antibody
        • Porin 31HL antibody
        • Porin 31HM antibody
        • VDAC antibody
        • VDAC-1 antibody
        • Vdac1 antibody
        • VDAC1_HUMAN antibody
        • Voltage dependent anion channel 1 antibody
        • Voltage dependent anion selective channel protein 1 antibody
        • Voltage-dependent anion-selective channel protein 1 antibody
        • YNL055C antibody
        • YNL2441C antibody
        see all

      Images

      • Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.

        Predicted band size: 30 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (33)

      Publishing research using ab110326? Please let us know so that we can cite the reference in this datasheet.

      ab110326 has been referenced in 33 publications.

      • Young DJ  et al. 40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling. Nat Commun 12:2976 (2021). PubMed: 34016977
      • Hijazi I  et al. Retrograde signaling mediates an adaptive survival response to endoplasmic reticulum stress. J Cell Sci N/A:N/A (2020). WB . PubMed: 32005698
      • Tu CC  et al. STIM2 knockdown protects against ischemia/reperfusion injury through reducing mitochondrial calcium overload and preserving mitochondrial function. Life Sci 247:116560 (2020). PubMed: 31200000
      • Zhou J  et al. Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola. Viruses 12:N/A (2020). PubMed: 32093074
      • Ghosh S  et al. An essential role for cardiolipin in the stability and function of the mitochondrial calcium uniporter. Proc Natl Acad Sci U S A 117:16383-16390 (2020). PubMed: 32601238
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Candida albicans Cell lysate - other (Cytosolic and mitochondrial fractions)
      Gel Running Conditions
      Reduced Denaturing (4-12% precast gel)
      Loading amount
      15 µg
      Treatment
      Induced with 10 mM proline for 1 h
      Specification
      Cytosolic and mitochondrial fractions
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Fitz Gerald S. Silao

      Verified customer

      Submitted Apr 26 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control

      Inconclusive
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Permeabilization
      Yes - 0.3% Triton X-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      PROF. Chichung Lie

      Verified customer

      Submitted Aug 01 2013

      Question

      Dear Technical,



      Good day! We have a customer who has placed an order for the above antibody but it is in back order and will not be available before March. Our customer is wondering if there is any alternative antibodies that they can use instead, as they need this before the middle of Feb.



      I was wondering if you could advise me about any alternative antibody that can be used.



      I hope to hear from you soon.



      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 23 2012

      Answer

      Thank you for your enquiry.
      Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer would like to use the other product so please advice her/him to read the products datasheets carefully to see which one would be suitable.
      - ab110326: https://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
      - ab61273: https://www.abcam.com/vdac-porin-antibody-mitochondrial-marker-ab61273.html
      - ab96140: https://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Jan 23 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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