Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
![Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)](https://www.abcam.com/ps/products/110/ab110326/Images/ab110326-123629-anti-vdac1-porin-antibody-16g9e6bc4-mitochondrial-loading-control-western-blot.jpg "Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)")
Key features and details
- Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
See all VDAC1/Porin primary antibodies -
Description
Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110326 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
16G9E6BC4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. |
Target
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Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
Tissue specificity
Heart, liver and skeletal muscle. -
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family. -
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
Cellular localization
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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Database links
- SwissProt: P04840 Saccharomyces cerevisiae
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Alternative names
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
Images
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Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.
Predicted band size: 30 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab110326 has been referenced in 41 publications.
- Brettrager EJ et al. Sulfotransferase 4A1 activity facilitates sulfate-dependent cellular protection to oxidative stress. Sci Rep 12:1625 (2022). PubMed: 35102205
- Liu Y et al. Inokosterone from Gentiana rigescens Franch Extends the Longevity of Yeast and Mammalian Cells via Antioxidative Stress and Mitophagy Induction. Antioxidants (Basel) 11:N/A (2022). PubMed: 35204097
- Nývltová E et al. Coordination of metal center biogenesis in human cytochrome c oxidase. Nat Commun 13:3615 (2022). PubMed: 35750769
- Kemmerer ZA et al. UbiB proteins regulate cellular CoQ distribution in Saccharomyces cerevisiae. Nat Commun 12:4769 (2021). PubMed: 34362905
- Broeskamp F et al. Porin 1 Modulates Autophagy in Yeast. Cells 10:N/A (2021). PubMed: 34572064