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    products/primary-antibodies/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (23)

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Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
  • Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
  • Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
  • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
  • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
  • Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18229] to VE Cadherin - Intercellular Junction Marker
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Assay
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Peroxidase Activity Assay Kit (ab155895)
Protein
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Recombinant Mouse VE Cadherin protein (ab208314)
Primary
Product image
Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab225443)

View more associated products

Overview

  • Product name

    Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker
    See all VE Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR18229] to VE Cadherin - Intercellular Junction Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung, placenta, heart, kidney and spleen lysates; bEnd.3 whole cell lysate. ICC/IF: bEnd.3 cells. IP: Mouse lung whole cell lysate; bEnd.3 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18229
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab225442)
    • Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab225443)
    • Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse VE Cadherin protein (ab208314)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa).
ICC/IF (1)
1/1000.
IP
1/80.
Notes
WB
1/1000. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa).
ICC/IF
1/1000.
IP
1/80.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificity

    Endothelial tissues and brain.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localization

    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Target information above from: UniProt accession P33151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12562 Mouse
    • SwissProt: P55284 Mouse
    • Unigene: 21767 Mouse
    • Alternative names

      • 7B 4 antibody
      • 7B4 antibody
      • 7B4 antigen antibody
      • CADH5_HUMAN antibody
      • Cadherin 5 antibody
      • Cadherin 5 type 2 antibody
      • Cadherin 5, type 2 (vascular endothelium) antibody
      • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
      • cadherin, vascular endothelial antibody
      • cadherin, vascular endothelial, 1 antibody
      • Cadherin-5 antibody
      • Cadherin5 antibody
      • CD 144 antibody
      • CD144 antibody
      • CD144 antigen antibody
      • CDH 5 antibody
      • CDH5 antibody
      • CDH5 protein antibody
      • Endothelial specific cadherin antibody
      • FLJ17376 antibody
      • OTTHUMP00000174777 antibody
      • Vascular endothelial cadherin antibody
      • Vascular epithelium cadherin antibody
      • VE Cad antibody
      • VE-cadherin antibody
      • VEC antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

      Confocal image showing membrane staining on bEnd.3 cell line. 

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at  1/1000 dilution and Goat Anti-Mouse IgG  H&L  (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.

      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/500 dilution.

    • Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      All lanes : Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Mouse placenta lysate
      Lane 3 : bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 88 kDa
      Observed band size: 125,90 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.

    • Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      All lanes : Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 88 kDa
      Observed band size: 120,90 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.

    • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

      VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.

      Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

      Lane 1: Mouse lung whole cell lysate 10ug (Input).

      Lane 2: ab205336 IP in Mouse lung whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

       

      Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.

    • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

      VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.

      Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

      Lane 1: bEnd.3 whole cell lysate 10ug (Input).

      Lane 2: ab205336 IP in bEnd.3 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

    • Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
      Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (23)

    Publishing research using ab205336? Please let us know so that we can cite the reference in this datasheet.

    ab205336 has been referenced in 23 publications.

    • Zhang J  et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
    • Shen J  et al. Foxo1-induced miR-92b down-regulation promotes blood-brain barrier damage after ischaemic stroke by targeting NOX4. J Cell Mol Med 25:5269-5282 (2021). PubMed: 33955666
    • Hong N  et al. The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling. Cell Death Dis 12:393 (2021). PubMed: 33846290
    • Cao X  et al. MSC-derived exosomal lncRNA SNHG7 suppresses endothelial-mesenchymal transition and tube formation in diabetic retinopathy via miR-34a-5p/XBP1 axis. Life Sci 272:119232 (2021). PubMed: 33600866
    • Zhang H  et al. Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9. Pharm Biol 59:760-768 (2021). PubMed: 34517742
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse brain endothelial cel)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Mouse brain endothelial cel
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Yohanes Wibowo

    Verified customer

    Submitted Aug 23 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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