Recombinant Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
![Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-252591-anti-ve-cadherin-antibody-epr18229-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18229] to VE Cadherin - Intercellular Junction Marker
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker
See all VE Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR18229] to VE Cadherin - Intercellular Junction Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung, placenta, heart, kidney and spleen lysates; bEnd.3 whole cell lysate. ICC/IF: bEnd.3 cells. IP: Mouse lung whole cell lysate; bEnd.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18229 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa).
|
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| ICC/IF | (1) |
1/1000.
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| IP |
1/80.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa). |
|
ICC/IF
1/1000. |
|
IP
1/80. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
Tissue specificity
Endothelial tissues and brain. -
Sequence similarities
Contains 5 cadherin domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 12562 Mouse
- SwissProt: P55284 Mouse
- Unigene: 21767 Mouse
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Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing membrane staining on bEnd.3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.
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![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-252595-anti-ve-cadherin-antibody-epr18229-western-blot.jpg)
Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)All lanes : Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse placenta lysate
Lane 3 : bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 88 kDa
Observed band size: 125,90 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
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![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-252594-anti-ve-cadherin-antibody-epr18229-western-blot.jpg)
Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)All lanes : Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 88 kDa
Observed band size: 120,90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
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![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-252590-anti-ve-cadherin-antibody-epr18229-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
-
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-252589-anti-ve-cadherin-antibody-epr18229-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: bEnd.3 whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in bEnd.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)](https://www.abcam.com/ps/products/205/ab205336/Images/ab205336-9-benefits-of-recombinant-antibodies.png)
Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (23)
ab205336 has been referenced in 23 publications.
- Zhang J et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
- Shen J et al. Foxo1-induced miR-92b down-regulation promotes blood-brain barrier damage after ischaemic stroke by targeting NOX4. J Cell Mol Med 25:5269-5282 (2021). PubMed: 33955666
- Hong N et al. The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling. Cell Death Dis 12:393 (2021). PubMed: 33846290
- Cao X et al. MSC-derived exosomal lncRNA SNHG7 suppresses endothelial-mesenchymal transition and tube formation in diabetic retinopathy via miR-34a-5p/XBP1 axis. Life Sci 272:119232 (2021). PubMed: 33600866
- Zhang H et al. Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9. Pharm Biol 59:760-768 (2021). PubMed: 34517742