Anti-VEGF Receptor 2 antibody (ab11939)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2
- Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-VEGF Receptor 2 antibody
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 2 -
Host species
Rabbit -
Specificity
The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types. -
Tested applications
Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).
-
Positive control
- IHC: Human spleen Flow Cyt: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate and HDLEC (Human dermal lymphatic endothelial cell line) whole cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody is affinity purified from rabbit sera. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
|
| IP |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| Neutralising |
Use at an assay dependent concentration. PubMed: 17991870
|
|
| WB | (1) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
|
| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
|
IP
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. PubMed: 17991870 |
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa. |
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
Use at an assay dependent concentration. |
Target
-
Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
see all -
Alternative names
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
Images
-
Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
-
Western blot - Anti-VEGF Receptor 2 antibody (ab11939)All lanes : Anti-VEGF Receptor 2 antibody (ab11939)
Lane 1 : recombinant human soluble VEGFR-2
Lane 2 : recombinant native VEGFR-2
Secondary
All lanes : anti-rabbit AP-conjugated antibody
Predicted band size: 151 kDaSamples were loaded in 10% SDS-PAGE under reducing conditions.
-
Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.
-
Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.
-
Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
-
Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (48)
ab11939 has been referenced in 48 publications.
- Grillo E et al. A novel variant of VEGFR2 identified by a pan-cancer screening of recurrent somatic mutations in the catalytic domain of tyrosine kinase receptors enhances tumor growth and metastasis. Cancer Lett 496:84-92 (2021). PubMed: 33035615
- Li P et al. Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB. J Transl Med 19:4 (2021). PubMed: 33407591
- Liu W et al. Ganoderma triterpenoids attenuate tumour angiogenesis in lung cancer tumour-bearing nude mice. Pharm Biol 58:1061-1068 (2020). PubMed: 33161828
- Liu H et al. Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells. Autophagy 16:2140-2155 (2020). PubMed: 31959090
- Hou K et al. Bone mesenchymal stem cell-derived exosomal microRNA-29b-3p prevents hypoxic-ischemic injury in rat brain by activating the PTEN-mediated Akt signaling pathway. J Neuroinflammation 17:46 (2020). PubMed: 32014002