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  1. Link

    products/primary-antibodies/vegf-receptor-2-antibody-ab11939.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 antibody (ab11939)

  • Datasheet
  • SDS
Reviews (3)Q&A (4)References (48)

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Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
  • Western blot - Anti-VEGF Receptor 2 antibody (ab11939)
  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
  • Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2
  • Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-VEGF Receptor 2 antibody [EPRER16Y] (ab134191)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description

    Rabbit polyclonal to VEGF Receptor 2
  • Host species

    Rabbit
  • Specificity

    The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types.
  • Tested applications

    Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).

  • Positive control

    • IHC: Human spleen Flow Cyt: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate and HDLEC (Human dermal lymphatic endothelial cell line) whole cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    This antibody is affinity purified from rabbit sera.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)
  • sELISA pair antibody

    • Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture.

IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 17991870
WB (1)
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture.

IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 17991870
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3791 Human
    • Entrez Gene: 16542 Mouse
    • Entrez Gene: 25589 Rat
    • Omim: 191306 Human
    • SwissProt: P35968 Human
    • SwissProt: P35918 Mouse
    • SwissProt: O08775 Rat
    • Unigene: 479756 Human
    • Unigene: 285 Mouse
    • Unigene: 88869 Rat
    see all
  • Alternative names

    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Western blot - Anti-VEGF Receptor 2 antibody (ab11939)
    Western blot - Anti-VEGF Receptor 2 antibody (ab11939)
    All lanes : Anti-VEGF Receptor 2 antibody (ab11939)

    Lane 1 : recombinant human soluble VEGFR-2
    Lane 2 : recombinant native VEGFR-2

    Secondary
    All lanes : anti-rabbit AP-conjugated antibody

    Predicted band size: 151 kDa



    Samples were loaded in 10% SDS-PAGE under reducing conditions.

  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.

  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.

  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)
    Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)

    Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (48)

Publishing research using ab11939? Please let us know so that we can cite the reference in this datasheet.

ab11939 has been referenced in 48 publications.

  • Grillo E  et al. A novel variant of VEGFR2 identified by a pan-cancer screening of recurrent somatic mutations in the catalytic domain of tyrosine kinase receptors enhances tumor growth and metastasis. Cancer Lett 496:84-92 (2021). PubMed: 33035615
  • Li P  et al. Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB. J Transl Med 19:4 (2021). PubMed: 33407591
  • Liu W  et al. Ganoderma triterpenoids attenuate tumour angiogenesis in lung cancer tumour-bearing nude mice. Pharm Biol 58:1061-1068 (2020). PubMed: 33161828
  • Liu H  et al. Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells. Autophagy 16:2140-2155 (2020). PubMed: 31959090
  • Hou K  et al. Bone mesenchymal stem cell-derived exosomal microRNA-29b-3p prevents hypoxic-ischemic injury in rat brain by activating the PTEN-mediated Akt signaling pathway. J Neuroinflammation 17:46 (2020). PubMed: 32014002
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

IHC - Wholemount abreview for Anti-VEGF Receptor 2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E10.5 embryo)
Specification
E10.5 embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sophie Astrof

Verified customer

Submitted Dec 25 2019

Western blot abreview for Anti-VEGF Receptor 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (Recombinant protein)
Gel Running Conditions
Reduced Denaturing
Loading amount
1 µg
Specification
Recombinant protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 24 2019

IHC - Wholemount abreview for Anti-VEGF Receptor 2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (whole embryo)
Specification
whole embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sophie Astrof

Verified customer

Submitted Dec 11 2019

Question

Actually I found the PO number: xxxxxxx.

Read More

Abcam community

Verified customer

Asked on Sep 11 2012

Answer

We have located the order associated with this purchase of ab11939 .It was purchased in May, 2008, which is well beyond the 6 month guarantee period we offer on all our products.

Is it possible that over the years, others in your lab have used the antibody, which could account for the low volume?

I hope this is helpful. Please contact us again if you have any further questions.

Read More

Abcam Scientific Support

Answered on Sep 11 2012

Question

Actually I found the PO number: xxxxxxx.

Read More

Abcam community

Verified customer

Asked on Sep 10 2012

Answer

Thanks for following up with me regarding the PO number for ab11939.

Unfortunately I have not found this number to correspond to any orders in our system. Also I have searched our last 2 years of orders for ab11939 and not found any from UC Riverside.

Does the PO information you found regarding this order have an associated order date?

Thanks!

Read More

Abcam Scientific Support

Answered on Sep 10 2012

Question

we used this antibody with human protein expressing cells and used another antibody recognizing the extracellular domain of VEGFR-2 as control which gave the expected result. The signal by ab11939 was not detectable. We have a lot of experience in ip and Western and do not perform such experiments for the first time

Read More

Abcam community

Verified customer

Asked on Nov 11 2005

Answer

Thank you for your email. Ab11939 has been tested for application in WB and IP with human samples, and our policy is that we guarantee that the product will work as stated on the online datasheet. If not, and the product has been purchased in the past 90 days, we will offer a replacement or refund. In order to investigate this, it would be very helpful if you could provide details regarding the protocol that was used. Also, please indicate the batch number (located on the vial) or the Abcam order number or purchase order number that was used. Thank you, and have a nice weekend.

Read More

Abcam Scientific Support

Answered on Nov 11 2005

Question

does this antibody immunoprecipitate also, I tested a first one, ab11939, from you which did not precipitate although it said it does so. I am eagerly looking for a good antibody directed at the intracellular domain that is useful for ip

Read More

Abcam community

Verified customer

Asked on Nov 07 2005

Answer

Thank you for your email. Yes, ab23734 has been tested for application in both IP and Western blotting. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. For ab11939, the antibody is guaranteed to work in IP with human samples. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=ip&pageconfig=technical&intAbID=11939&mode=questionaire

Read More

Abcam Scientific Support

Answered on Nov 07 2005

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