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  1. Link

    products/primary-antibodies/vegf-receptor-2-antibody-ab39638.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 antibody (ab39638)

  • Datasheet
  • SDS
Reviews (3)Q&A (9)References (18)

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Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Anti-VEGF Receptor 1 + VEGF Receptor 2 antibody (ab36844)

View more associated products

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description

    Rabbit polyclonal to VEGF Receptor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.

  • Positive control

    • WB: SK-OV3 cell lysate. IHC-P: Human brain tissue and stomach cancer tissue; Rat spinal cord tissue. ICC/IF: HepG2 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 µg/ml.
WB (1)
1/500 - 1/1000. Predicted molecular weight: 151 kDa.
IHC-P (1)
1/50 - 1/100.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 151 kDa.
IHC-P
1/50 - 1/100.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3791 Human
    • Entrez Gene: 16542 Mouse
    • Entrez Gene: 25589 Rat
    • Omim: 191306 Human
    • SwissProt: P35968 Human
    • SwissProt: P35918 Mouse
    • SwissProt: O08775 Rat
    • Unigene: 479756 Human
    • Unigene: 285 Mouse
    • Unigene: 88869 Rat
    see all
  • Alternative names

    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

    Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of human brain tissue stained with ab39638. The image on the right is blocked with the synthesized peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of human stomach cancer tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of rat spinal cord tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.

  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
    ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Anti-VEGF Receptor 2 antibody (ab39638) (Anti-VEGF Receptor 2 antibody) + HeLa cell lysate

    Predicted band size: 151 kDa

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab39638? Please let us know so that we can cite the reference in this datasheet.

ab39638 has been referenced in 18 publications.

  • Gehmeyr J  et al. Disabling VEGF-Response of Purkinje Cells by Downregulation of KDR via miRNA-204-5p. Int J Mol Sci 22:N/A (2021). PubMed: 33671638
  • Qian JJ  et al. Expression of VEGF-A Signaling Pathway in Cartilage of ACLT-induced Osteoarthritis Mouse Model. J Orthop Surg Res 16:379 (2021). PubMed: 34127028
  • Virumbrales-Muñoz M  et al. Organotypic primary blood vessel models of clear cell renal cell carcinoma for single-patient clinical trials. Lab Chip 20:4420-4432 (2020). PubMed: 33103699
  • Zhou BY  et al. Nintedanib inhibits keloid fibroblast functions by blocking the phosphorylation of multiple kinases and enhancing receptor internalization. Acta Pharmacol Sin 41:1234-1245 (2020). PubMed: 32327724
  • Wang M  et al. Glioma exosomal microRNA-148a-3p promotes tumor angiogenesis through activating the EGFR/MAPK signaling pathway via inhibiting ERRFI1. Cancer Cell Int 20:518 (2020). PubMed: 33117083
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-VEGF Receptor 2 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (human stomach cancer cell derived tissue)
Loading amount
30 µg
Specification
human stomach cancer cell derived tissue
Treatment
20uM cannabinoid antagonist
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 09 2013

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