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Anti-VEGF Receptor 2 antibody (ab39638)

Reviews (3)Q&A (9)References (21)
Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies

  • Description

    Rabbit polyclonal to VEGF Receptor 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.

  • Positive control

    • WB: SK-OV3 cell lysate. IHC-P: Human brain tissue and stomach cancer tissue; Rat spinal cord tissue. ICC/IF: HepG2 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 µg/ml.
WB (1)
1/500 - 1/1000. Predicted molecular weight: 151 kDa.
IHC-P (1)
1/50 - 1/100.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 151 kDa.
IHC-P
1/50 - 1/100.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

    Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of human brain tissue stained with ab39638. The image on the right is blocked with the synthesized peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of human stomach cancer tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)

    Immunohistochemical analysis of rat spinal cord tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.

  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
    Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
    ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
    Anti-VEGF Receptor 2 antibody (ab39638) (Anti-VEGF Receptor 2 antibody) + HeLa cell lysate

    Predicted band size: 151 kDa

References (21)

Publishing research using ab39638? Please let us know so that we can cite the reference in this datasheet.

ab39638 has been referenced in 21 publications.

  • Ma C  et al. Curcumol repressed cell proliferation and angiogenesis via SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer. Front Pharmacol 13:1044115 (2022). PubMed: 36467048
  • Luo Y  et al. Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway. Front Mol Biosci 8:652443 (2021). PubMed: 34746227
  • Gehmeyr J  et al. Disabling VEGF-Response of Purkinje Cells by Downregulation of KDR via miRNA-204-5p. Int J Mol Sci 22:N/A (2021). PubMed: 33671638
  • Qian JJ  et al. Expression of VEGF-A Signaling Pathway in Cartilage of ACLT-induced Osteoarthritis Mouse Model. J Orthop Surg Res 16:379 (2021). PubMed: 34127028
  • Zhou BY  et al. Nintedanib inhibits keloid fibroblast functions by blocking the phosphorylation of multiple kinases and enhancing receptor internalization. Acta Pharmacol Sin 41:1234-1245 (2020). PubMed: 32327724
View all Publications for this product

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestine (tissue))
Permeabilization
Yes - Citrate pH 6, pressure cooker 13min
Specification
Intestine (tissue)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Rebeca Figueira

Verified customer

Submitted Dec 20 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart, with a myocardial infarction)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
Heart, with a myocardial infarction
Blocking step
10% goat serum and 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Anita Chandler

Verified customer

Submitted Jul 28 2015

Western blot abreview for Anti-VEGF Receptor 2 antibody

 Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (human stomach cancer cell derived tissue)
Loading amount
30 µg
Specification
human stomach cancer cell derived tissue
Treatment
20uM cannabinoid antagonist
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 09 2013

Question

ab39638 not working in ICC on stimulated HuVec cells. ab9530 is giving excellent results.

Read More
Answer

Thank you for your reply. That is super news. Would it be possible for you to submit these images to our datasheets via our Abreview system? We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews Thank you for your time and effort, all the best in your future research.

Read More

Question

Antibody not working in ICC

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. I look forward to receiving your images of ab39638. As requested, I have issued a free of charge replacement of ab9530. I am out of the office next week so I would encourage you to phone us on Wednesday to check the order has been shipped. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I hope the new products works well, and I wish you the best of luck with your research.

Read More

Question

Thank you very much, Just received the ab ab36993. Will set that up on Wed (10-19-11) on human tissue One question does this ab cross react with mouse LYVE1?

Read More
Answer

Thank you for your enquiry. To our knowledge ab36993 has not been tested for reactivity to mouse LYVE1. It may cross react with mouse protein, however, as it is a polyclonal product raised against a full length immunogen protein. I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

Thank you very much for working with me on this. yes I would like to replace ab39638 with rabbit anti-LYVE1 ab36993.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab36993. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More

Question

That is what I was worried about. the new lot you sent me gave identical results as the previous lot number. this would be great if we were sure we were getting VEGFR2 staining but again both lots look more like where we would find VEGFR3

Read More
Answer

I am sorry to hear the replacement vial of ab39638 did not improve things. Again, I would be happy to offer a different primary antibody, credit or refund. Please let me know how you wish to proceed.

Read More

Question

Thank you very much for sending to me the new lot number for  VEGFR2. Would you please tell me, are this two lots bleeds from the same rabbit and purified at different times or are they from two different rabbits altogether?

Read More
Answer

Yes, they are from the same rabbit.

Read More

Question

Thank you very much. Do you think as well with the new lot number I could receive another vial from the same lot number that I have been trying? lot 762406. I would like to try the new lot heads up with the old lot number and have run out of that vial trying to get this to work out.

Read More
Answer

Thanks for your enquiry. I can only send out 1 vial of antibody to replace the 1 vial that was not working as expected. Please note as well that we have sold out of lot 762406 for ab39638. Please let me know if you need anything further.

Read More

1-10 of 12 Abreviews or Q&A

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