Recombinant Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)
![Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)](https://www.abcam.com/ps/products/221/ab221679/Images/ab221679-307751-anti-vegf-receptor-2-antibody-epr21884-290-western-blot.jpg "Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-VEGF Receptor 2 antibody [EPR21884-290]
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat placenta and lung lysates; bEnd.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21884-290 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 180-230 kDa (predicted molecular weight: 152 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 180-230 kDa (predicted molecular weight: 152 kDa). |
Target
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Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 285 Mouse
- Unigene: 88869 Rat
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Alternative names
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
Images
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Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)All lanes : Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679) at 1/1000 dilution
Lane 1 : Mouse placenta lysate at 20 µg
Lane 2 : Rat placenta lysate at 20 µg
Lane 3 : Rat lung lysate at 20 µg
Lane 4 : Mouse lung lysate at 20 µg
Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 180-230 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 3 minutes; Lane 3: 37 seconds; Lane 4: 59 seconds; Lane 5: 15 seconds
The molecular mass observed is consistent with the literature (PMID: 23354168 ; PMID: 20606037).
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![Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)](https://www.abcam.com/ps/products/221/ab221679/Images/ab221679-2-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab221679 has been referenced in 1 publication.
- Song J et al. Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33846786