Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)

Submit a review Submit a question
Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
  • ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
  • Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRER16Y] to VEGF Receptor 2 - BSA and Azide free
  • Suitable for: ELISA, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free
    See all VEGF Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPRER16Y] to VEGF Receptor 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment aa 1-800. The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta lysate.

  • General notes

    ab226160 is the carrier-free version of ab134191.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa).
IP
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa).
IP
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
      Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
      Anti-VEGF Receptor 2 antibody [EPRER16Y] (ab134191) at 1/500 dilution + Human placenta lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 151 kDa
      Observed band size: 210,230 kDa why is the actual band size different from the predicted?



      This data was developed using ab134191, the same antibody clone in a different buffer formulation.

       

    • ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
      ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)

      This data was developed using ab134191, the same antibody clone in a different buffer formulation.

      ELISA analysis of Hu VEGFR2 recombinant protein at 1000 ng/mL with ab134191. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)
      Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free (ab226160)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226160? Please let us know so that we can cite the reference in this datasheet.

    ab226160 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab226160.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com