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    products/primary-antibodies/vegf-receptor-2-phospho-y951-antibody-ab38473.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
  • Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2 (phospho Y951)
  • Suitable for: ELISA, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-VEGF Receptor 2 (phospho Y951) antibody
    See all VEGF Receptor 2 primary antibodies
  • Description

    Rabbit polyclonal to VEGF Receptor 2 (phospho Y951)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y951).

  • Positive control

    • WB: SK-OV3 cell extract. IHC-P: Breast carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab38473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 151 kDa).
IHC-P
1/50 - 1/100.
ICC/IF (2)
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 151 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3791 Human
    • Entrez Gene: 16542 Mouse
    • Entrez Gene: 25589 Rat
    • Omim: 191306 Human
    • SwissProt: P35968 Human
    • SwissProt: P35918 Mouse
    • SwissProt: O08775 Rat
    • Unigene: 479756 Human
    • Unigene: 285 Mouse
    • Unigene: 88869 Rat
    see all
  • Alternative names

    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
    ab38473 at a 1:50 dilution staining VEGF Receptor 2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : untreated.

    Right image : treated with phosphopeptide.

  • Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
    Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
    All lanes : Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)

    Lane 1 : SK-OV-3 (Human ovarian cancer cell line) cell lysate. Treated with phosphopeptide
    Lane 2 : SK-OV-3 (Human ovarian cancer cell line) cell lysate

    Predicted band size: 151 kDa
    Observed band size: 230 kDa why is the actual band size different from the predicted?



    VEGFR2 is translated into a 150 kDa protein. Glycosylation of the extracellular domain results in the mature form at the cell surface which migrates at 230 kDa via western blot.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab38473? Please let us know so that we can cite the reference in this datasheet.

ab38473 has been referenced in 8 publications.

  • Desjarlais M  et al. Tyrosine-Protein Phosphatase Non-receptor Type 9 (PTPN9) Negatively Regulates the Paracrine Vasoprotective Activity of Bone-Marrow Derived Pro-angiogenic Cells: Impact on Vascular Degeneration in Oxygen-Induced Retinopathy. Front Cell Dev Biol 9:679906 (2021). PubMed: 34124069
  • Li C  et al. HIF-1a/VEGF signaling-mediated epithelial-mesenchymal transition and angiogenesis is critically involved in anti-metastasis effect of luteolin in melanoma cells. Phytother Res 33:798-807 (2019). PubMed: 30653763
  • Huang M  et al. Apatinib affect VEGF-mediated cell proliferation, migration, invasion via blocking VEGFR2/RAF/MEK/ERK and PI3K/AKT pathways in cholangiocarcinoma cell. BMC Gastroenterol 18:169 (2018). PubMed: 30400838
  • Zhao G  et al. The Soluble VEGF Receptor sFlt-1 Contributes to Impaired Neovascularization in Aged Mice. Aging Dis 8:287-300 (2017). PubMed: 28580185
  • Kapur S  et al. Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis. Bioeng Transl Med 2:81-91 (2017). WB . PubMed: 28516164
  • Zhang W  et al. Combined aspirin and apatinib treatment suppresses gastric cancer cell proliferation. Oncol Lett 14:5409-5417 (2017). PubMed: 29142602
  • Koyama Y  et al. Endothelin-1 increases the expression of VEGF-R1/Flt-1 receptors in rat cultured astrocytes through ETB receptors. J Neurochem 130:759-69 (2014). PubMed: 24862165
  • Sangaletti S  et al. Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Res 68:9050-9 (2008). IHC-P ; Mouse . PubMed: 18974151

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 (phospho Y951) antibody

Average
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (endothelial cells)
Specification
endothelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1%Triton/0.1%NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 18 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 (phospho Y951) antibody

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (tumor cell)
Specification
tumor cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X 100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 19 2007

Question

Thank you for your email and suggestions. May I request to proceed with a refund for the antibody? Thanks Regards

Read More

Abcam community

Verified customer

Asked on Dec 28 2011

Answer

We are happy to refund your purchase. However it seems that you have purchased this antibody from our distributor in Australia. Could you please contact them and ask them to request a refund from Abcam for you. If the product was in 6 month guarantee period I can then refund the whole purchase. I am sorry I can’t refund you directly because we do not have your purchase order number details. Please contact Sapphire Bioscience in Australia they will be able to help you.

Read More

Abcam Scientific Support

Answered on Dec 28 2011

Question

Please see below an enquiry from a customer that has already been in contact with you directly. Thank you for your email. According to the datasheet, this antibody is supposed to work on paraffin sections. However, it was not stated as to whether an antigen retrieval should be performed. As such, I have done an antigen retrieval but I was unable to get the antibody to work. He told me he has tried the solutions offered but they did not work for him. Hope to hear from you soon, Kind regards,

Read More

Abcam community

Verified customer

Asked on Dec 22 2011

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. I can further offer you a replacement vial from different lot or a different antibody against the same target. Let me know how you would like to proceed. Looking forward to hearing from you soon.

Read More

Abcam Scientific Support

Answered on Dec 22 2011

Question

LOT NUMBER GR55595 ORDER NUMBER 4450020066 DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse kidney cryosections Mouse kidney paraffin sections PRIMARY ANTIBODY Primary antibody at 1:100 dilution was used with incubation at 4C overnight DETECTION METHOD Immunofluorescence POSITIVE AND NEGATIVE CONTROLS USED Positive staining was expected in the kidney sections ANTIBODY STORAGE CONDITIONS -20C FIXATION OF SAMPLE 4%PFA fixation overnight for paraffin sections and 1hr for cryosections ANTIGEN RETRIEVAL Antigen retrieval performed on paraffin sections using citrate buffer boiling method for 10mins PERMEABILIZATION STEP 0.1% Tween20/PBS for 5 mins BLOCKING CONDITIONS 1%BSA for 30mins SECONDARY ANTIBODY Detected using anti-rabbit Alexa 488 (Invitrogen)at 1:200 dilution for 2 hrs at room temperature HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Nil ADDITIONAL NOTES Nil

Read More

Abcam community

Verified customer

Asked on Dec 21 2011

Answer

Thank you for your enquiry regarding ab38473 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. The protocol looks fine to me however I would like to ask few questions which would help me identify the source of the problem; - ab38473 is a phospho specific antibody which will detect the phosphorylated VEGFR2. We have successfully used human breast carcinoma tissue for QC test of this antibody. I would suggest using human breast cancerous tissues sections as positive control. I would also suggest checking the following publication; "VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis" http://www.mendeley.com/research/vegf-receptor2-y951-signaling-role-adapter-molecule-tsad-tumor-angiogenesis/# - Optimization of antigen retrieval step can also help. I would suggest trying the 5, 15 and 20 minute’s incubation time also. - For frozen section acetone or etahnol fixatives are highly recommended. I hope these suggestions will be helpful. Let me know if the results do not improve; I will be happy to assist you further.

Read More

Abcam Scientific Support

Answered on Dec 21 2011

Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal in the position of 190k SAMPLE SD-rat endothelial cell PRIMARY ANTIBODY dilution 1: 1000 in TBST with 2% BSA DETECTION METHOD ECL-plus POSITIVE AND NEGATIVE CONTROLS USED no positive control, because this product only test in human cell or tissue, no rat cell. can you provide me some positive control extraction? SAMPLE PREPARATION extracted by 8M urea AMOUNT OF PROTEIN LOADED 30ug ELECTROPHORESIS/GEL CONDITIONS 7.5% SDS_PAGE TRANSFER AND BLOCKING CONDITIONS nitrocellial mambrane, 75 V, 3hours in cold room transfer buffer contain 20% Met,0.2% SDS, block: 2% BSA, 5% dry milk overnight SECONDARY ANTIBODY goat anti-rabbit dilution 1:4000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no milk

Read More

Abcam community

Verified customer

Asked on Aug 31 2007

Answer

Thank you for your patience in awaiting a response. As mentioned previously the suggested positive control is rat brain. Briefly the lysate was prepared in the following way: The test was made with whole rat brain tissue. 1) Place the tissue in a homogenizing tube which was on ice. Add extraction buffer (here RIPA buffer was used) in the tube to cover the tissue (2x more than tissue by volume). 2) Homogenize the tissue with a homogenizer on ice. 3) Centrifuge 8,000rpm for 30 min at 4 C. Take the supernatant. 4) Assay protein concentration. Apply 20 or 30 ug protein/per line on SDS-PAGE. I hope that you find this helpful. Should you continue to experience problems then please get back in touch with me again.

Read More

Abcam Scientific Support

Answered on Aug 31 2007

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