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    products/primary-antibodies/vegf-receptor-3-antibody-epr22293-14-ab243232.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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RecombinantRabMAb

Recombinant Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)
  • Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-VEGF Receptor 3 antibody [EPR22293-14]
    See all VEGF Receptor 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEL whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22293-14
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 175, 125, 80 kDa (predicted molecular weight: 146 kDa).
Notes
WB
1/1000. Detects a band of approximately 175, 125, 80 kDa (predicted molecular weight: 146 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • Tissue specificity

    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • Involvement in disease

    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35916 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2324 Human
    • Omim: 136352 Human
    • SwissProt: P35916 Human
    • Unigene: 646917 Human
    • Alternative names

      • EC 2.7.10.1 antibody
      • flt 4 antibody
      • FLT-4 antibody
      • FLT4 antibody
      • FLT41 antibody
      • Fms related tyrosine kinase 4 antibody
      • Fms-like tyrosine kinase 4 antibody
      • LMPH1A antibody
      • PCL antibody
      • Soluble VEGFR3 variant 1 antibody
      • Soluble VEGFR3 variant 2 antibody
      • Soluble VEGFR3 variant 3 antibody
      • Tyrosine protein kinase receptor FLT4 antibody
      • Tyrosine-protein kinase receptor FLT4 antibody
      • Vascular endothelial growth factor receptor 3 antibody
      • Vascular endothelial growth factor receptor 3 precursor antibody
      • VEGF R3 antibody
      • VEGFR 3 antibody
      • VEGFR-3 antibody
      • VEGFR3 antibody
      • VGFR3_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)
      Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)
      All lanes : Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232) at 1/1000 dilution

      Lane 1 : HEL (human erythroleukemia erythroblast cell line) whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 146 kDa
      Observed band size: 125,175,80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a higher sensitivity ECL substrate.

      The molecular weight observed is consistent with the literatures (PMID: 18538738, PMID: 7692369, PMID: 14648657).

      Negative control: MCF7 (PMID: 18538738).

    • Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)
      Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab243232? Please let us know so that we can cite the reference in this datasheet.

    ab243232 has not yet been referenced specifically in any publications.

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