Recombinant Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)
![Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)](https://www.abcam.com/ps/products/243/ab243565/Images/ab243565-333738-ab243565.jpg "Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free
See all VEGF Receptor 3 primary antibodies -
Description
Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEL whole cell lysate.
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General notes
ab243565 is the carrier-free version of ab243232.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22293-14 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 175, 125, 80 kDa (predicted molecular weight: 146 kDa).
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 175, 125, 80 kDa (predicted molecular weight: 146 kDa). |
Target
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Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity. -
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain. -
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2324 Human
- Omim: 136352 Human
- SwissProt: P35916 Human
- Unigene: 646917 Human
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Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Images
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Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)All lanes : Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232) at 1/1000 dilution
Lane 1 : HEL (human erythroleukemia erythroblast cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 146 kDa
Observed band size: 125,175,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
The molecular weight observed is consistent with the literatures (PMID: 18538738, PMID: 7692369, PMID: 14648657).
Negative control: MCF7 (PMID: 18538738).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243232).
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![Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)](https://www.abcam.com/ps/products/243/ab243565/Images/ab243565-2-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab243565 has not yet been referenced specifically in any publications.
