Recombinant Anti-VEGF Receptor 3 antibody [EPR24718-58] (ab300403)
![Western blot - Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)](https://www.abcam.com/ps/products/300/ab300403/Images/AB300403-488236-anti-vegf-receptor-3-epr2471858-western-blot-2.jpg "Western blot - Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24718-58] to VEGF Receptor 3
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-VEGF Receptor 3 antibody [EPR24718-58]
See all VEGF Receptor 3 primary antibodies -
Description
Rabbit monoclonal [EPR24718-58] to VEGF Receptor 3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEnd.3 whole cell lysates, mouse lung and liver tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24718-58 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 153 kDa (predicted molecular weight: 146 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 153 kDa (predicted molecular weight: 146 kDa). |
Target
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Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity. -
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain. -
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 14257 Mouse
- SwissProt: P35917 Mouse
- Unigene: 3291 Mouse
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Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Images
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Western blot - Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)All lanes : Anti-VEGF Receptor 3 antibody [EPR24718-58] (ab300403) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Predicted band size: 146 kDa
Observed band size: 153 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
The identity of the lower MW band at approximately 50 kDa is unknown.
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![Western blot - Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)](https://www.abcam.com/ps/products/300/ab300403/Images/AB300403-488234-anti-vegf-receptor-3-epr2471858-western-blot-1.jpg)
Western blot - Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)All lanes : Anti-VEGF Receptor 3 antibody [EPR24718-58] (ab300403) at 1/1000 dilution
Lane 1 : bEnd.3 (mouse brain endothelioma) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 146 kDa
Observed band size: 153 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
Negative control: NIH/3T3 (PMID: 16984732)
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The faint band detected at ~200 kDa likely represents a glycosylated form of VEGFR3 (PMID: 20697430). -
![Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)](https://www.abcam.com/ps/products/300/ab300403/Images/AB300403-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-VEGF Receptor 3 antibody [EPR24718-58] (AB300403)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300403 has not yet been referenced specifically in any publications.