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    products/primary-antibodies/vegfa-antibody-ep1176y-c-terminal-ab52917.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF
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RecombinantRabMAb

Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (138)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)
  • Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody [EP1176Y] (ab52917)
  • Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)
  • Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
  • Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1176Y] to VEGFA - C-terminal
  • Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free PE

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Overview

  • Product name

    Anti-VEGFA antibody [EP1176Y] - C-terminal
    See all VEGFA primary antibodies
  • Description

    Rabbit monoclonal [EP1176Y] to VEGFA - C-terminal
  • Host species

    Rabbit
  • Specificity

    We do not guarantee IHC-P for mouse.

    This antibody fails to detect endogenous natural samples in WB. We recommend to use ab214424 for WB.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human VEGFA aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P15692

  • Positive control

    • Purchase matching WB positive control:Recombinant Human VEGFA protein
    • IHC-P: Human kidney tissue. ICC/IF: NIH/3T3 cells. Flow Cyt (intra): NIH/3T3 cells.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1176Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Angiogenic factors ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-VEGFA antibody [EP1176Y] - BSA and Azide free (ab185238)
    • Biotin Anti-VEGFA antibody [EP1176Y] (ab195902)
    • Alexa Fluor® 488 Anti-VEGFA antibody [EP1176Y] (ab206886)
    • Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887)
    • Alexa Fluor® 594 Anti-VEGFA antibody [EP1176Y] (ab206888)
    • PE Anti-VEGFA antibody [EP1176Y] (ab209439)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human VEGFA protein (ab55566)
    • Recombinant mouse VEGFA protein (ab9954)
  • Related Products

    • Human VEGF ELISA Kit (ab100662)
    • Human VEGF ELISA Kit (ab100663)
    • Mouse VEGF ELISA Kit (ab100751)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (6)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

See IHC antigen retrieval protocols.

ICC/IF (1)
1/250 - 1/500.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/1000 dilution.

Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

See IHC antigen retrieval protocols.

ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/1000 dilution.

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Target information above from: UniProt accession P15692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7422 Human
    • Entrez Gene: 22339 Mouse
    • Omim: 192240 Human
    • SwissProt: P15692 Human
    • SwissProt: Q96KJ0 Human
    • SwissProt: Q00731 Mouse
    • Unigene: 73793 Human
    • Unigene: 282184 Mouse
    • Alternative names

      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling VEGFA with purified ab52917 at 1:100 dilution (2.96 µg/ml).

      Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0.

      Negative control shown in inset.

    • Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody [EP1176Y] (ab52917)
      Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody [EP1176Y] (ab52917)

      Purified ab52917 staining VEGF in NIH/3T3 (Mouse embryo fibroblast cell line) cells.

      The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with purified ab52917 at 5 μg/ml and ab195884, at 1/250 dilution, overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Rabbit Alexa Fluor®488 secondary (ab150081) at 2 μg/ml (shown in green).

      Nuclear DNA was labeled in blue with DAPI.

    • Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
      Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

      Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained withunpurified ab52917 (red line). 

      The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab52917, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1 µg/1x106 cells used under the same conditions. 

      Unlabeled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

      This antibody gave a positive signal in NIH/3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used at 1/100 dilution. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] (ab52917)This image is courtesy of an anonymous Abreview

      Unpurified ab52917 staining VEGF in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 4% BSA + 5% serum in PBST) for 14 hours at 4°C. An Alexa Fluor® 546-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    • Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
      Flow Cytometry (Intracellular) - Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

      Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling VEGFA with purified ab52917 at 1/30 dilution (10µg/ml) (red). 

      Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). 

      Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
      Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (138)

    Publishing research using ab52917? Please let us know so that we can cite the reference in this datasheet.

    ab52917 has been referenced in 138 publications.

    • Ma L  et al. Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone. Int J Mol Med 47:N/A (2021). PubMed: 33448319
    • Bhattacharjee J  et al. Physical activity differentially regulates VEGF, PlGF, and their receptors in the human placenta. Physiol Rep 9:e14710 (2021). PubMed: 33463910
    • Chen K  et al. Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair. Int J Nanomedicine 16:371-381 (2021). PubMed: 33469291
    • Wan B  et al. GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris. Aging (Albany NY) 13:7067-7083 (2021). PubMed: 33621952
    • Zhu W  et al. Macrophage migration inhibitory factor facilitates the therapeutic efficacy of mesenchymal stem cells derived exosomes in acute myocardial infarction through upregulating miR-133a-3p. J Nanobiotechnology 19:61 (2021). PubMed: 33639970
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGFA antibody [EP1176Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Brain Microvascular Endothelial Cells (HBMEC)
    Permeabilization
    Yes - Triton 0.1x
    Specification
    Human Brain Microvascular Endothelial Cells (HBMEC
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Pepa Carrion-Navarro

    Verified customer

    Submitted Jul 18 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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