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Cardiovascular Angiogenesis Growth Factors VEGF VEGF
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RecombinantRabMAb

Recombinant Anti-VEGFA antibody [EPR20705] (ab214424)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (7)

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Western blot - Anti-VEGFA antibody [EPR20705] (ab214424)
  • Anti-VEGFA antibody [EPR20705] (ab214424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20705] to VEGFA
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-VEGFA antibody [EPR20705]
    See all VEGFA primary antibodies
  • Description

    Rabbit monoclonal [EPR20705] to VEGFA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal vessel lysate; HeLa, HUVEC, SH-SY5Y, Neuro-2a, bEND.3, C6 and RAW 264.7 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20705
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human VEGFA protein (ab55566)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Target information above from: UniProt accession P15692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7422 Human
    • Entrez Gene: 22339 Mouse
    • Entrez Gene: 83785 Rat
    • Omim: 192240 Human
    • SwissProt: P15692 Human
    • SwissProt: Q96KJ0 Human
    • SwissProt: Q00731 Mouse
    • SwissProt: P16612 Rat
    • Unigene: 73793 Human
    • Unigene: 282184 Mouse
    • Unigene: 1923 Rat
    see all
  • Alternative names

    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Images

  • Western blot - Anti-VEGFA antibody [EPR20705] (ab214424)
    Western blot - Anti-VEGFA antibody [EPR20705] (ab214424)
    All lanes : Anti-VEGFA antibody [EPR20705] (ab214424) at 1/1000 dilution

    Lane 1 : Human fetal vessel lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 6 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
    Lane 7 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDM/TBST

    Exposure time : Lanes 1-4/7-8: 3 minutes; Lane 5: 41 seconds; Lane 6: 15 seconds.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Anti-VEGFA antibody [EPR20705] (ab214424)
    Anti-VEGFA antibody [EPR20705] (ab214424)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (7)

Publishing research using ab214424? Please let us know so that we can cite the reference in this datasheet.

ab214424 has been referenced in 7 publications.

  • Zhou W  et al. Rab27a deletion impairs the therapeutic potential of endothelial progenitor cells for myocardial infarction. Mol Cell Biochem 476:797-807 (2021). PubMed: 33095380
  • Niu R  et al. Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy. Aging (Albany NY) 13:8643-8664 (2021). PubMed: 33714952
  • Zhang P  et al. Distinct mRNAs in Cancer Extracellular Vesicles Activate Angiogenesis and Alter Transcriptome of Vascular Endothelial Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33921957
  • Zhao Y  et al. Activated mesangial cells induce glomerular endothelial cells proliferation in rat anti-Thy-1 nephritis through VEGFA/VEGFR2 and Angpt2/Tie2 pathway. Cell Prolif 54:e13055 (2021). PubMed: 33987885
  • Song J  et al. Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33846786
  • Zhou Y  et al. Chemically modified curcumin (CMC2.24) alleviates osteoarthritis progression by restoring cartilage homeostasis and inhibiting chondrocyte apoptosis via the NF-?B/HIF-2a axis. J Mol Med (Berl) 98:1479-1491 (2020). PubMed: 32860098
  • Ji L  et al. Immunosubunit ß5i Knockout Suppresses Neovascularization and Restores Autophagy in Retinal Neovascularization by Targeting ATG5 for Degradation. Invest Ophthalmol Vis Sci 61:30 (2020). PubMed: 33369639

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