Anti-VEGFA antibody [VG-1] (ab1316)

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Anti-VEGFA antibody [VG-1] (ab1316)


Mouse monoclonal


Application:　ELISA, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB


Reactivity:　Mouse, Rat, Rabbit, Dog, Human


100 µg¥73,000　国内在庫ございます


https://www.abcam.co.jp/vegfa-antibody-vg-1-ab1316.html　




追加のご質問等ございましたら、お知らせください。

The Active human VEGF full length protein (ab9571) has been tested on HUVECs and is known to stimulate proliferation of these cells, which shows that the GAG sites are present and active.
The recombinant human VEGF165 is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. Unfortunately, we cannot be certain on where exactly the GAG sites are present. However, because ab9571 has been tested on HUVECs and does stimulate proliferation we would think that the GAG sites are present and active. Please be aware that anyhow we cannot guarantee it.
The Anti-VEGF antibody [VG-1] (ab1316) is able to detect Active human VEGF full length protein (ab9571) because ab9571 is VEGF 165 and ab1316 is able to detect VEGF 165 (please refer to the antibody datasheet for more information).

Thank you for contacting us.


I will gladly help you to find a suitable antibody for your experiment. However, it seems that both targets are likely to produce an abundance of side bands in WB as both occur in many isoforms. In addition, even when we may have a lot of antibodies for the targets, it looks like HepG2 cells have not been used in many cases (especially with the BRCA1 target). In order to distinguish the signal from the side bands, I would like to suggest to tag the protein or to run a positive control in form of the recombinant proteins in order to check how well the antibody is picking up the signal.

The two antibodies I have picked are the following

Click here (or use the following: https://www.abcam.com/VEGFA-antibody-VG-1-ab1316.html). ab1316 Anti-VEGF antibody [VG-1] has been used with HepG2 cells, and has received good customer rating.

https://www.abcam.com/BRCA1-antibody-ab79401.html (or use the following: https://www.abcam.com/BRCA1-antibody-ab79401.html).

ab79401 Anti-BRCA1 antibodyseems to produce a nice clear signal.



I hope this information is helpful to you. Please let me know if these two antibodies are suitable for you.
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Thank you for contacting us. To our knowledge, the epitope for the VG-1 clone has not been mapped. While we cannot say for certain which isoforms will be recognized in rat, bands are observed at ˜27 and 31 kDa in rat dermal epithelium samples. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for your answer.

I have made now an alignment of the sequence you provided,with the three sequences we know the ab1316 does react with. Indeed the sequences are in identical in the the main parts. Please see here by yourself:
hhttp://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120410-153045-0967-732581-pg

I can therefore confirm that the epitope is contained in your recombinantly expressed protein.
The antibody ab1316 will therefore most likely recognise your recombinantly expressed protein, if the expression is correct.

I hope this information is helpful. Please do not hesitate to contact us again should you have any concern or question.

Thank you for your inquiry.

We have not determined the exact epitope of the antibody ab1316. I can however confirm that this antibody does recognize the human121, 165 and 189 VEGF isoforms (SwissProt: P15692) and I would expect therefore that it would recognize also the recombinant VEGF you are expressing, if the recombinant VEGF from your source does not contain major modifications. The antibody is also guaranteed to work in Western blot.

In summary, I would expect the antibody to work on your protein, although we can not guarantee it.

As an alternative, you might can also look at a polyclonal antibody, which he has several epitopes, such as the ab14078, the ab17696or the ab9570.

https://www.abcam.com/VEGF-antibody-ab14078.html (or use the following: https://www.abcam.com/VEGF-antibody-ab14078.html).
https://www.abcam.com/VEGF-antibody-ab17696.html (or use the following: https://www.abcam.com/VEGF-antibody-ab17696.html).
https://www.abcam.com/VEGFA-antibody-ab9570.html (or use the following: https://www.abcam.com/VEGFA-antibody-ab9570.html).

I hope this information is helpful. Please do not hesitate to let me know if you have any other questions or concerns.

Thank you for your suggestion. I will will put it forward as a suggestion but I think ab1316 would not be the best candidate as having performed sequence analysis of the isoforms this antibody has been shown to recognise (121, 165 and 189) with that of 165b, it seems unlikely that it would be specific for either 165 of 165b. From the file I have attached, The sequence are as follows: P15692-8 isoform 165b P15692-9 isoform 121 P15692-11 isoform 165 P15692-13 isoform 189 From this it can be seen that the 6 amino acid residues of the C-terminal which differentiates between the 165 and 165b isoform are not present at all in isoform 121. I would therefore suspect that the antibody is not binding in this region. I will however have a look through the rest of our catalogue to see if we have any other anti-VEGF antibodies which may be more likely to bind 165 specifically and put these forward for testing. I will let you know of my progress. If you have any further questions or suggestion we would be very pleased to hear them.

Thank you for contacting us in regards to Anti-VEGF antibody [VG-1] (ab1316). I’m afraid we currently have no information on whether this antibody would recognise the VEGF isoform 165b. The full length recombinant protein is used as the immunogen and in depth epitope mapping has as yet not been performed. However, as the only difference between the isoforms 165 and 165b is the final 6 C-terminal amino acids I think there is a good chance the antibody recognises a different region. I cannot guarantee it though. We have two antibodies in our catalogue where the VEGF 165b is specifically recognised: ab14994 is a mouse monoclonal which has been used to detect human and rat protein. ab90719 is a rabbit polyclonal which has been used to detect human VEGF 165b. Depending on the applications you intend to use one of these antibodies may be suitable for you. I hope this information has been of help. If you would like any further information please do let me know.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with ab32152 and the order number 984908. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Could you confirm that you need ab1316 VEGF antibody; this antibody is not a VEGF receptor 2 specific antibody? I can also suggest trying ethanol as fixative which is far less harsh than acetone; PFA is not a common fixative used in IHC-Fr so you may consider trying ethanol.   I hope this will help.