Recombinant Anti-VEGFD antibody [EPR8457] (ab155288)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8457] to VEGFD
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 APC Biotin Carrier Free HRP PE

Product name

Anti-VEGFD antibody [EPR8457]
See all VEGFD primary antibodies
Description

Rabbit monoclonal [EPR8457] to VEGFD
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human VEGFD. The exact sequence is proprietary.
Database link: O43915
(Peptide available as ab189506)
Positive control
- Raji, HT-1080, MCF-7, and Caco-2 cell lysates. Human heart and lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8457
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 whole cell lysate (ab29537)
- Raji whole cell lysate (ab30124)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P		1/100 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/200.

Notes
Flow Cyt (Intra) 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.

Function

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Tissue specificity

Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
Sequence similarities

Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications

Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Cellular localization

Secreted.
Target information above from: UniProt accession O43915 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2277 Human
- Entrez Gene: 14205 Mouse
- Entrez Gene: 360457 Rat
- Omim: 300091 Human
- SwissProt: O43915 Human
- SwissProt: P97946 Mouse
- SwissProt: O35251 Rat
- Unigene: 11392 Human
- Unigene: 297978 Mouse
- Unigene: 10796 Rat
see all
Alternative names
- c-fos induced growth factor antibody
- c-fos induced growth factor (vascular endothelial growth factor D) antibody
- c-fos induced growth factor antibody
- c-fos-induced growth factor antibody
- FIGF antibody
- Vascular endothelial growth factor D antibody
- Vascular endothelial growth factor D precursor antibody
- Vascular endothelial growth factor D precursor antibody
- VEGF D antibody
- VEGF-D antibody
- VEGFD antibody
- VEGFD_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control: PBS in place of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab155288 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution

Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
Lane 2 : HT1080 (human fibrosarcoma) whole cell lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

Diluting and blocking buffer: 5% NFDM/TBST
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution (Unpurified) + GST-Tagged human VEGFD (aa 1 to 354) recombinant protein

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 40 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
Flow Cytometry (Intracellular) - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in the mouse cell line NIH/3T3 (mouse embryo) by intracellular flow cytometry.Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/2000 dilution

Lane 1 : Rat lung tissue lysate
Lane 2 : NIH/3T3 (mouse embryo) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

Diluting and blocking buffer: 5% NFDM/TBST
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/1000 dilution

Lane 1 : Raji lysate
Lane 2 : HT-1080 lysate
Lane 3 : MCF-7 lysate
Lane 4 : Caco-2 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155288 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab155288showing +ve staining.
Anti-VEGFD antibody [EPR8457] (ab155288)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab155288? Please let us know so that we can cite the reference in this datasheet.

ab155288 has been referenced in 10 publications.

Dachy G et al. Novel COL4A1-VEGFD gene fusion in myofibroma. J Cell Mol Med 25:4387-4394 (2021). PubMed: 33830670
Zhao L et al. Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia. J Diabetes Res 2020:6286571 (2020). PubMed: 32104713
Wan X et al. microRNA-382 suppresses the progression of pancreatic cancer through the PI3K/Akt signaling pathway by inhibition of Anxa3. Am J Physiol Gastrointest Liver Physiol N/A:N/A (2020). PubMed: 32463333
Niu Y et al. CCL25 promotes the migration and invasion of non-small cell lung cancer cells by regulating VEGF and MMPs in a CCR9-dependent manner. Exp Ther Med 19:3571-3580 (2020). PubMed: 32346420
Sun B et al. Colorectal cancer exosomes induce lymphatic network remodeling in lymph nodes. Int J Cancer 145:1648-1659 (2019). PubMed: 30734278
Harris K et al. Immunohistochemical Features of Epithelial-Mesenchymal Transition in Feline Oral Squamous Cell Carcinoma. Vet Pathol 56:826-839 (2019). PubMed: 31331247
Zhao T et al. The siRNA silencing of DcR3 expression induces Fas ligand-mediated apoptosis in HepG2 cells. Exp Ther Med 15:4370-4378 (2018). PubMed: 29725377
Jiang J et al. Immunohistochemical characterization of lymphangiogenesis-related biomarkers in primary and recurrent gliomas: A STROBE compliant article. Medicine (Baltimore) 97:e12458 (2018). PubMed: 30278527
Song H et al. Dietary oleuropein inhibits tumor angiogenesis and lymphangiogenesis in the B16F10 melanoma allograft model: a mechanism for the suppression of high-fat diet-induced solid tumor growth and lymph node metastasis. Oncotarget 8:32027-32042 (2017). PubMed: 28410190
Hong H et al. TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway. BMC Cancer 16:240 (2016). IHC . PubMed: 26992854

Customer reviews and Q&As

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Question

Anti-VEGFD antibody [EPR8457] (ab155288), is there any cross reactivity with VEGFc?

Abcam community

Verified customer

Asked on Mar 01 2013

Answer

Ab155288 has not been tested specifically for cross-reactivity with VEGFC, however I aligned the immunogen sequence against the human VEGFC sequence and there is no significant similarity between the sequences. So, we would not predict the antibody to cross react with VEGFC.

Abcam Scientific Support

Answered on Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (10)

Customer reviews and Q&As

Post-translational
modifications