Anti-VGLUT2 antibody [8G9.2] (ab79157)
![Immunohistochemistry - Free Floating - Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94655-anti-vglut2-antibody-8g92-immunohistochemistry.jpg "Immunohistochemistry - Free Floating - Anti-VGLUT2 antibody [8G9.2] (ab79157)")
Key features and details
- Mouse monoclonal [8G9.2] to VGLUT2
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-VGLUT2 antibody [8G9.2]
See all VGLUT2 primary antibodies -
Description
Mouse monoclonal [8G9.2] to VGLUT2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FrFl, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Rat VGLUT2.
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Positive control
- Floating mouse brain tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.328% Sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8G9.2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 22064485
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| IHC-P | (3) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
1/1000.
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| IHC-FrFl | (1) |
Use at an assay dependent concentration.
|
| WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
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| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 22064485 |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/1000. |
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IHC-FrFl
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
Tissue specificity
Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus. -
Sequence similarities
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
Developmental stage
Expressed in fetal brain. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 140919 Mouse
- Entrez Gene: 84487 Rat
- SwissProt: Q8BLE7 Mouse
- SwissProt: Q9JI12 Rat
- Unigene: 256618 Mouse
- Unigene: 19372 Rat
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Alternative names
- Differentiation associated BNPI antibody
- Differentiation associated Na dependent inorganic phosphate cotransporter antibody
- Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
see all
Images
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Immunohistochemistry - Free Floating - Anti-VGLUT2 antibody [8G9.2] (ab79157)ab79157, at 1/300 dilution, staining VGLUT2 by Immunohistochemistry using ABC with NiDAB on floating mouse brain tissue sections.
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![Immunocytochemistry/ Immunofluorescence - Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94654-anti-vglut2-antibody-8g92-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VGLUT2 antibody [8G9.2] (ab79157)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomImmunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.
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![Western blot - Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94653-anti-vglut2-antibody-8g92-western-blot.jpg)
Western blot - Anti-VGLUT2 antibody [8G9.2] (ab79157)All lanes : Anti-VGLUT2 antibody [8G9.2] (ab79157) at 1 µg/ml
Lane 1 : Rat Cortex Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab79157 has been referenced in 40 publications.
- Velasco ER et al. PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamus. Nat Commun 13:4374 (2022). PubMed: 35902577
- Mercado NM et al. The BDNF Val66Met polymorphism (rs6265) enhances dopamine neuron graft efficacy and side-effect liability in rs6265 knock-in rats. Neurobiol Dis 148:105175 (2021). PubMed: 33188920
- Humburg BA et al. Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice. Addict Biol 26:e13005 (2021). PubMed: 33538103
- Florido A et al. Sex differences in fear memory consolidation via Tac2 signaling in mice. Nat Commun 12:2496 (2021). PubMed: 33941789
- Gilissen SRJ et al. Reconsidering the Border between the Visual and Posterior Parietal Cortex of Mice. Cereb Cortex 31:1675-1692 (2021). PubMed: 33159207