Recombinant Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
![Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-387265-anti-vitamin-d-receptor-antibody-epr4552-chip-grade-western-blot-hela-u937-shsy5y-lysates.jpg "Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade
- Suitable for: WB, IP, ChIP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade
See all Vitamin D Receptor primary antibodies -
Description
Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, U-937, T-47D and SKBR-3 cell lysates; Rat and mouse kidney tissue lysates. ChIP: Chromatin prepared from T-47D cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4552 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/1000 - 1/10000. |
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| IP |
1/30.
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| ChIP |
Use 5 µg for 25 µg of chromatin.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/1000 - 1/10000. |
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IP
1/30. |
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ChIP
Use 5 µg for 25 µg of chromatin. |
Target
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Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all -
Alternative names
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
Images
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Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Vitamin D Receptor knockout HeLa lysate
Lane 3 : U-937 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaLanes 1-4: Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-8-AntiVitamin-D-Receptor-antibody-EPR4552ChIP.jpg)
ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 5 µg of ab109234 (red), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (grey). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.
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![Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-250041-anti-vitamin-d-receptor-antibody-epr4552-western-blot.jpg)
Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-298394-anti-vitamin-d-receptor-antibody-epr4552-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
Lane 2(+): T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysateAb109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
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![Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-250042-anti-vitamin-d-receptor-antibody-epr4552-western-blot.jpg)
Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-250043-anti-vitamin-d-receptor-antibody-epr4552-western-blot.jpg)
Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-121905-anti-vitamin-d-receptor-antibody-epr4552-western-blot.jpg)
Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (unpurified)
Lane 1 : T47D cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
![Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)](https://www.abcam.com/ps/products/109/ab109234/Images/ab109234-10-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab109234 has been referenced in 23 publications.
- Yang Y et al. Vitamin D protects glomerular mesangial cells from high glucose-induced injury by repressing JAK/STAT signaling. Int Urol Nephrol 53:1247-1254 (2021). PubMed: 33942213
- Cai Q et al. Inflammation-Associated Senescence Promotes Helicobacter pylori-Induced Atrophic Gastritis. Cell Mol Gastroenterol Hepatol 11:857-880 (2021). PubMed: 33161156
- Bass JJ et al. The mechanisms of skeletal muscle atrophy in response to transient knockdown of the vitamin D receptor in vivo. J Physiol 599:963-979 (2021). PubMed: 33258480
- Paukovcekova S et al. Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor a Expression in Osteosarcoma Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 32916897
- Hu Z et al. VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis. Cell Death Dis 11:73 (2020). PubMed: 31996668