Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)
Key features and details
- Mouse monoclonal [1H10] to VLDL Receptor/VLDL-R
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-VLDL Receptor/VLDL-R antibody [1H10]
See all VLDL Receptor/VLDL-R primary antibodies -
Description
Mouse monoclonal [1H10] to VLDL Receptor/VLDL-R -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6.60
Constituents: 0.58% Sodium chloride, 0.0292% EDTA, 0.82% Sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Produced in a VLDL Receptor/VLDL-R knockout mouse. -
Primary antibody notes
Blocks apoE4 binding. -
Clonality
Monoclonal -
Clone number
1H10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. -
Tissue specificity
Abundant in heart and skeletal muscle; also ovary and kidney; not in liver. -
Involvement in disease
Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsUbiquitinated at Lys-839 by MYLIP leading to degradation. -
Cellular localization
Membrane. Membrane > clathrin-coated pit. - Information by UniProt
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Database links
- Entrez Gene: 7436 Human
- Entrez Gene: 22359 Mouse
- Omim: 192977 Human
- SwissProt: P98155 Human
- SwissProt: P98156 Mouse
- Unigene: 370422 Human
- Unigene: 4141 Mouse
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Alternative names
- FLJ35024 antibody
- Very low density lipoprotein receptor antibody
- Very low-density lipoprotein receptor antibody
see all
Images
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Overlay histogram showing HEK293 cells stained with ab75591 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75591, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Flow Cytometry - Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)This image is courtesy of an anonymous Abreview
Flow cytometry analysis of Human THP-1 cell lines, staining VLDL Receptor/VLDL-R (red histogram) with ab75591.
Cells were taken from suspension culture and washed with PBS/BSA before fixation. Cells were blocked and permeabilized in 10% donkey serum/0.3 M glycine/0.1% BSA/0.1% Tween-20 in PBS for 20 mins at room temperature. The sample was incubated with the primary antibody (2 µg/106 cells in 0.1% BSA/0.1% Tween-20 in PBS) for 30 minutes at 22°C. A FITC-conjugated donkey anti-mouse polyclonal IgG (30 µg/ml) was used as the secondary antibody.
Gating Strategy: FSC/SSC
Datasheets and documents
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Datasheet download
References (2)
ab75591 has been referenced in 2 publications.
- Kothari V et al. ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages. Arterioscler Thromb Vasc Biol 41:1854-1873 (2021). PubMed: 33882688
- Gardner JK et al. Lipid-laden partially-activated plasmacytoid and CD4(-)CD8a(+) dendritic cells accumulate in tissues in elderly mice. Immun Ageing 11:11 (2014). Flow Cyt ; Mouse . PubMed: 25089147