Anti-VMA2 antibody [13D11B2] (ab113684)
![Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)](https://www.abcam.com/ps/products/113/ab113684/Images/ab113684-126378-anti-vma2-antibody-13d11b2-western-blot.jpg "Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)")
Key features and details
- Mouse monoclonal [13D11B2] to VMA2
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-VMA2 antibody [13D11B2] -
Description
Mouse monoclonal [13D11B2] to VMA2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Yeast membranes
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
13D11B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
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| WB |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| Notes |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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Database links
- Entrez Gene: 852424 Saccharomyces cerevisiae
- SwissProt: P16140 Saccharomyces cerevisiae
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Alternative names
- V-ATPase 57 kDa subunit antibody
- V-ATPase subunit B antibody
- V-type proton ATPase subunit B antibody
see all
Images
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Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)Anti-VMA2 antibody [13D11B2] (ab113684) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113684 has been referenced in 1 publication.
- Belgareh-Touzé N et al. Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy. Autophagy 13:114-132 (2017). WB ; Saccharomyces cerevisiae . PubMed: 27846375