Recombinant Anti-Von Willebrand Factor antibody [EPR25069-131] (ab287962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25069-131] to Von Willebrand Factor
- Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Von Willebrand Factor antibody [EPR25069-131]
See all Von Willebrand Factor primary antibodies -
Description
Rabbit monoclonal [EPR25069-131] to Von Willebrand Factor -
Host species
Rabbit -
Specificity
This antibody does not react with cleaved von Willebrand antigen 2 as the immunogen is located at the C-terminus
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Tested applications
Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse platelet, Rat platelet lysates. IHC-P: Rat spleen, Mouse spleen, Mouse colon and Rat lung, Mouse pancreas tumor tissues. IHC-Fr: Mouse lung, Rat liver and Rat lung, Mouse liver tissues. Flow Cyt: Mouse blood cells. ICC/IF: bEnd.3 (mouse brain endothelial cell) cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25069-131 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab287962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50.
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WB |
1/1000. Predicted molecular weight: 309 kDa.
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IHC-Fr |
1/500.
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Flow Cyt |
1/500.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/50. |
WB
1/1000. Predicted molecular weight: 309 kDa. |
IHC-Fr
1/500. |
Flow Cyt
1/500. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Database links
- Entrez Gene: 22371 Mouse
- Entrez Gene: 116669 Rat
- SwissProt: Q8CIZ8 Mouse
- SwissProt: Q62935 Rat
- Unigene: 22339 Mouse
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Alternative names
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling Von Willebrand Factor with ab287962 at 1/100 dilution (5.58 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody used at 1/1000 dilution (2µg/ml) (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µg/ml)(Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing cytoplasmic staining in bEnd.3 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). -
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tumor tissue labelling VonWillebrand Factor with ab287962 at 1/50 (11.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Cytoplasmic staining on endothelial of mouse pancreas tumor. The section was incubated with ab287962 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling VonWillebrand Factor with ab287962 at 1/500 (1.11 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the endothelial cells in mouse liver is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
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All lanes : Anti-Von Willebrand Factor antibody [EPR25069-131] (ab287962) at 1/1000 dilution
Lane 1 : Mouse platelet lysate
Lane 2 : Rat platelet lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 309 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBSTBands below 300kDa were VWF cleavage fragments(PMID: 21909423)
Exposure time: 70 seconds
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Flow cytometric analysis of Mouse blood cells cells labelling VonWillebrand Factor with ab287962 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab287962. Then stained with anti-CD41 conjugated to APC.Gated on viable cells.
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Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling VonWillebrand Factor with ab287962 at 1/50 (11.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Cytoplasmic staining on endothelial cells of rat lung. The section was incubated with ab287962 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh) tissue labeling VonWillebrand Factor with ab287962 at 1/500 (1.11 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the vascular endothelial cells in rat lung is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling VonWillebrand Factor with ab287962 at 1/50 (11.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Cytoplasmic staining on endothelial cells of mouse colon. The section was incubated with ab287962 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling VonWillebrand Factor with ab287962 at 1/500 (1.11 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the endothelial cells in rat liver is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling VonWillebrand Factor with ab287962 at 1/50 (11.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Cytoplasmic staining on megakaryocyte and platelets of mouse spleen. The section was incubated with ab287962 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling VonWillebrand Factor with ab287962 at 1/500 (1.11 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the vascular endothelial cells in mouse lung is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
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Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling VonWillebrand Factor with ab287962 at 1/50 (11.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Cytoplasmic staining on megakaryocyte and platelets of rat spleen. The section was incubated with ab287962 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab287962 has been referenced in 1 publication.
- Gao F et al. SESN1 attenuates the Ox‑LDL‑induced inflammation, apoptosis and endothelial‑mesenchymal transition of human umbilical vein endothelial cells by regulating AMPK/SIRT1/LOX1 signaling. Mol Med Rep 25:N/A (2022). PubMed: 35293601