Recombinant Anti-VPAC2 antibody [SP235] (ab183334)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-6-AntiVPAC2-antibody-SP235-IHC-Rat-spleen.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP235] to VPAC2
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VPAC2 antibody [SP235]
See all VPAC2 primary antibodies -
Description
Rabbit monoclonal [SP235] to VPAC2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver, hepatocellular carcinoma, liver cirrhosis, Human tonsil, Mouse spleen, and Rat spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP235 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/100.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/100. |
Target
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Function
This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C. -
Tissue specificity
Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7434 Human
- Entrez Gene: 22355 Mouse
- Entrez Gene: 29555 Rat
- Omim: 601970 Human
- SwissProt: P41587 Human
- SwissProt: P41588 Mouse
- SwissProt: P35000 Rat
- Unigene: 585052 Human
see all -
Alternative names
- Helodermin-preferring VIP receptor antibody
- PACAP type III receptor antibody
- PACAP-R-3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-5-AntiVPAC2-antibody-SP235-IHC-Mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-4-AntiVPAC2-antibody-SP235-IHC-Human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-3-antiVPAC2antibodySP235-flowcytometry-Jurkat-human.jpg)
Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling VPAC2 with purified ab183334 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-216742-anti-vpac2-antibody-sp235-flow-cytometry.jpg)
Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling VPAC2 using ab183334 (green) compare to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-216741-anti-vpac2-antibody-sp235-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling VPAC2 using ab183334 at a 1/100 dilution.
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![Anti-VPAC2 antibody [SP235] (ab183334)](https://www.abcam.com/ps/products/183/ab183334/Images/ab183334-7-benefits-of-recombinant-antibodies.png)
Anti-VPAC2 antibody [SP235] (ab183334)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183334 has been referenced in 1 publication.
- Asano S et al. Vasoactive intestinal peptide-VIPR2 signaling regulates tumor cell migration. Front Oncol 12:852358 (2022). PubMed: 36237322