Anti-VPH1 antibody [10D7A7B2] (ab113683)
![Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)](https://www.abcam.com/ps/products/113/ab113683/Images/ab113683-126377-anti-vph1-antibody-10d7a7b2-western-blot.jpg "Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)")
Key features and details
- Mouse monoclonal [10D7A7B2] to VPH1
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
Overview
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Product name
Anti-VPH1 antibody [10D7A7B2] -
Description
Mouse monoclonal [10D7A7B2] to VPH1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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General notes
This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
Clonality
Monoclonal -
Clone number
10D7A7B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
|
| ICC/IF |
Use a concentration of 20 µg/ml.
Heat induced antigen retrieval recommended. |
| Notes |
|---|
|
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
|
ICC/IF
Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended. |
Target
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Function
Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Sequence similarities
Belongs to the V-ATPase 116 kDa subunit family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Vacuole membrane. - Information by UniProt
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Database links
- Entrez Gene: 854444 Saccharomyces cerevisiae
- SwissProt: P32563 Saccharomyces cerevisiae
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Alternative names
- V-ATPase 95 kDa subunit antibody
- V-ATPase a 1 subunit antibody
- V-type proton ATPase subunit a antibody
see all
Images
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Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)All lanes : Anti-VPH1 antibody [10D7A7B2] (ab113683)
Lane 1 : Yeast whole cell lysate
Lane 2 : yeast membrane preparation
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab113683 has been referenced in 7 publications.
- Hose J et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
- Chen H et al. The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity. PLoS Genet 16:e1009046 (2020). PubMed: 33064727
- Karim MA et al. Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion. Dev Cell 47:80-97.e6 (2018). PubMed: 30269949
- Cavellini L et al. An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion. Nat Commun 8:15832 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28607491
- Dodgson SE et al. The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance. Genes Dev 30:2259-2271 (2016). PubMed: 27807036
- Simpkins JA et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). PubMed: 27142334