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RecombinantRabMAb

Recombinant Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)

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Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
  • Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
  • Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9225] to VPS4B/MIG1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free
    See all VPS4B/MIG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9225] to VPS4B/MIG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248775 is the carrier-free version of ab137027.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9225

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the AAA ATPase family.
    Contains 1 MIT domain.

  • Domain

    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • 8030489C12Rik antibody
      • Cell migration inducing 1 antibody
      • Cell migration-inducing gene 1 protein antibody
      • MGC116271 antibody
      • MIG1 antibody
      • Protein SKD1 antibody
      • Skd1 antibody
      • SKD1B antibody
      • Suppressor of K(+) transport growth defect 1 antibody
      • Suppressor of K+ transport defect 1 antibody
      • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
      • Vacuolar protein sorting 4 homolog B antibody
      • Vacuolar protein sorting 4b antibody
      • Vacuolar protein sorting-associated protein 4B antibody
      • Vacuolar protein sorting-associating protein 4B antibody
      • VPS4 2 antibody
      • VPS42 antibody
      • Vps4b antibody
      • VPS4B_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
      Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
      All lanes : Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa



      This data was developed using ab137027, the same antibody clone in a different buffer formulation.

    • Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
      Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)

      This data was developed using ab137027, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of permeabilized 293T cells labelling VPS4B/MIG1 with ab137027 at 1/10 dilution (red). Rabbit IgG negative control is green. 

       

       

    • Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
      Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248775? Please let us know so that we can cite the reference in this datasheet.

    ab248775 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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