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  1. Link

    products/primary-antibodies/vsv-g-tag-antibody-ab83196.pdf

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Tags & Cell Markers Epitope Tags Other Tags VSV-G Tag
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Anti-VSV-G tag antibody (ab83196)

  • Datasheet
Reviews (3) Submit a question

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Western blot - Anti-VSV-G tag antibody (ab83196)

    Key features and details

    • Rabbit polyclonal to VSV-G tag
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-VSV-G tag antibody [EPR12997] (ab183497)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-VSV-G tag antibody
      See all VSV-G tag primary antibodies
    • Description

      Rabbit polyclonal to VSV-G tag
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Full length protein corresponding to VSV-G tag aa 1 to the C-terminus.
      Database link: P04884

    • General notes

      0.2 µ filtered

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Other Tags
      • VSV-G Tag

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab83196 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.
    Notes
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.

    Target

    • Relevance

      Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
    • Alternative names

      • Vesicular stomatitis virus glycoprotein tag antibody
      • VSV epitope tag antibody
      • VSV tag antibody
      • VSV-G epitope tag antibody
      • VSVG antibody
      • vsvg tag antibody
      see all

    Images

    • Western blot - Anti-VSV-G tag antibody (ab83196)
      Western blot - Anti-VSV-G tag antibody (ab83196)Image is courtesy of an anonymous AbReview.
      All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution

      Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG)
      Lane 2 : Human HEK293 mock transfected (labelled -VSVG)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 10 kDa


      Exposure time: 10 seconds

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab83196? Please let us know so that we can cite the reference in this datasheet.

    ab83196 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-VSV-G tag antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (4T1)
    Permeabilization
    Yes - methanol
    Specification
    4T1
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. roberto de Antueno

    Verified customer

    Submitted Sep 03 2018

    Western blot abreview for Anti-VSV-G tag antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293 cell line)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    10 µg
    Treatment
    Transfected with pMD2.G or mock
    Specification
    HEK293 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 28 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VSV-G tag antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293 cell line)
    Permeabilization
    No
    Specification
    HEK293 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 28 2015

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    For licensing inquiries, please contact partnerships@abcam.com

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